ID   C6A14_DROME             Reviewed;         509 AA.
AC   Q9V4U7; A0A0B4LEW9;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   07-NOV-2018, sequence version 3.
DT   03-AUG-2022, entry version 159.
DE   RecName: Full=Probable cytochrome P450 6a14;
DE            EC=1.14.-.-;
DE   AltName: Full=CYPVIA14;
GN   Name=Cyp6a14; ORFNames=CG8687;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   CONCEPTUAL TRANSLATION.
RA   Nelson B.;
RL   Unpublished observations (SEP-2000).
CC   -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC       the breakdown of synthetic insecticides. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC       Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC       {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AE013599; AHN55997.1; -; Genomic_DNA.
DR   RefSeq; NP_001286199.1; NM_001299270.1.
DR   RefSeq; NP_610389.3; NM_136545.3.
DR   AlphaFoldDB; Q9V4U7; -.
DR   SMR; Q9V4U7; -.
DR   IntAct; Q9V4U7; 1.
DR   STRING; 7227.FBpp0302888; -.
DR   PaxDb; Q9V4U7; -.
DR   PRIDE; Q9V4U7; -.
DR   EnsemblMetazoa; FBtr0302331; FBpp0311645; FBgn0033302.
DR   EnsemblMetazoa; FBtr0345575; FBpp0311646; FBgn0033302.
DR   GeneID; 35835; -.
DR   KEGG; dme:Dmel_CG8687; -.
DR   UCSC; CG8687-RB; d. melanogaster.
DR   CTD; 35835; -.
DR   FlyBase; FBgn0033302; Cyp6a14.
DR   VEuPathDB; VectorBase:FBgn0033302; -.
DR   eggNOG; KOG0158; Eukaryota.
DR   HOGENOM; CLU_535951_0_0_1; -.
DR   InParanoid; Q9V4U7; -.
DR   OMA; GMYMFFK; -.
DR   OrthoDB; 467733at2759; -.
DR   PhylomeDB; Q9V4U7; -.
DR   BioGRID-ORCS; 35835; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 35835; -.
DR   PRO; PR:Q9V4U7; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0033302; Expressed in spermathecum and 22 other tissues.
DR   ExpressionAtlas; Q9V4U7; baseline and differential.
DR   Genevisible; Q9V4U7; DM.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..509
FT                   /note="Probable cytochrome P450 6a14"
FT                   /id="PRO_0000051870"
FT   BINDING         454
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   509 AA;  58233 MW;  05DE50F1FD149A37 CRC64;
     MLFTIALVGV VLGLAYSLHI KIFSYWKRKG VPHETPLPIV GNMRGIVKKY HFRDINQRIY
     KKFKGQGPIA GMYMFFKRTA LITDLDFIKQ VMIKDFSYFQ DRGAFTNPRD DPLTGHLFAL
     EGEEWRAMRH KLTPVFTSGK IKQMSKVIVD VGLRLGDAMD KAVKEAKVEE GNVEIKDLCA
     RFTTDVIGSC AFGLECNSLQ DPSAEFRQKG REIFTRRRHS TLVQSFIFTN ARLARKLRIK
     VLPDDLTQFF MSTVKNTVDY RLKNGIKRND FIEQMIELRA EDQEAAKKGQ GIDLSHGLTL
     EQMAAQAFVF FVAGFETSSS TMSLCLYELA LQPDIQQRLR EEIESVLANV DGGELNYDVL
     AQMTYLDQVL SETLRKHPLL PHLIRETTKD YQIPNSDIVL DKGILALIPV HNIHHDPEIY
     PEPEKFDPSR FDPEEVKNRH PMAYLPFGDG PRNCIGLRFG KIQAKIGLVS LLRRFKFSVS
     NRTDVPLIFS KKSFLLTTND GIYLKVERV