TX405_LYCSI
ID TX405_LYCSI Reviewed; 109 AA.
AC B6DCT7;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 25-MAY-2022, entry version 18.
DE RecName: Full=U4-lycotoxin-Ls1d {ECO:0000305};
DE Short=U4-LCTX-Ls1d {ECO:0000305};
DE AltName: Full=Toxin-like structure LSTX-C5;
DE Flags: Precursor;
OS Lycosa singoriensis (Wolf spider) (Aranea singoriensis).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Lycosoidea; Lycosidae; Lycosa.
OX NCBI_TaxID=434756;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Venom gland;
RX PubMed=19875276; DOI=10.1016/j.zool.2009.04.001;
RA Zhang Y., Chen J., Tang X., Wang F., Jiang L., Xiong X., Wang M., Rong M.,
RA Liu Z., Liang S.;
RT "Transcriptome analysis of the venom glands of the Chinese wolf spider
RT Lycosa singoriensis.";
RL Zoology 113:10-18(2010).
CC -!- FUNCTION: Enhances the high-affinity desensitization of human P2RX3
CC purinoceptors. {ECO:0000250|UniProtKB:B3EWH0}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:19875276}.
CC -!- DOMAIN: The toxin is composed of 2 domains: a highly rigid N-terminal
CC inhibitor cystine knot (knottin) domain and a rather flexible C-
CC terminal linear cationic cytotoxin domain that forms amphiphilic alpha-
CC helices. {ECO:0000250|UniProtKB:B3EWH0}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000250|UniProtKB:B3EWH0}.
CC -!- SIMILARITY: Belongs to the neurotoxin 19 (CSTX) family. 05 (U4-Lctx)
CC subfamily. {ECO:0000305}.
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DR EMBL; EU926021; ACI41353.1; -; mRNA.
DR EMBL; FM864025; CAS03623.1; -; mRNA.
DR AlphaFoldDB; B6DCT7; -.
DR SMR; B6DCT7; -.
DR ArachnoServer; AS000970; U4-lycotoxin-Ls1d.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR019553; Spider_toxin_CSTX_knottin.
DR InterPro; IPR011142; Spider_toxin_CSTX_Knottin_CS.
DR Pfam; PF10530; Toxin_35; 1.
DR PROSITE; PS60029; SPIDER_CSTX; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Knottin; Secreted; Signal; Toxin.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..44
FT /evidence="ECO:0000250"
FT /id="PRO_0000401687"
FT CHAIN 45..109
FT /note="U4-lycotoxin-Ls1d"
FT /id="PRO_0000401688"
FT REGION 45..88
FT /note="Knottin domain"
FT /evidence="ECO:0000250|UniProtKB:B3EWH0"
FT REGION 89..108
FT /note="Linear cationic cytotoxin domain"
FT /evidence="ECO:0000250|UniProtKB:B3EWH0"
FT DISULFID 48..63
FT /evidence="ECO:0000250|UniProtKB:B3EWH0"
FT DISULFID 55..72
FT /evidence="ECO:0000250|UniProtKB:B3EWH0"
FT DISULFID 62..88
FT /evidence="ECO:0000250|UniProtKB:B3EWH0"
FT DISULFID 74..86
FT /evidence="ECO:0000250|UniProtKB:B3EWH0"
SQ SEQUENCE 109 AA; 12472 MW; 51F8139861478E5C CRC64;
MKVLVLFSVL FLTLFSYSST EAMDEFDSDA EEDMLSLMAN EQVRAKACTP RLHDCSHDRH
SCCRGELFKD VCYCFYPEGE DKTEVCSCQQ PKSHKYIEKV VDKARTVVG