TX4BC_DINQU
ID TX4BC_DINQU Reviewed; 28 AA.
AC C0HJH6;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-SEP-2014, sequence version 1.
DT 25-MAY-2022, entry version 14.
DE RecName: Full=M-poneritoxin-Dq4b/U1-poneritoxin-Dq4c/U1-poneritoxin-Dq4d {ECO:0000303|PubMed:26805882};
DE Short=M-PONTX-Dq4b/U1-PONTX-Dq4c/U1-PONTX-Dq4d {ECO:0000303|PubMed:26805882};
DE AltName: Full=Dinoponeratoxin Dq-3162/Dq-3163/Dq-3178 {ECO:0000303|PubMed:24157790};
DE AltName: Full=Poneratoxin {ECO:0000305};
OS Dinoponera quadriceps (South American ant).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Ponerinae; Ponerini; Dinoponera.
OX NCBI_TaxID=609295;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP SYNTHESIS, OXIDATION AT MET-20, AND AMIDATION AT ALA-28.
RC TISSUE=Venom;
RX PubMed=24157790; DOI=10.1016/j.jprot.2013.10.017;
RA Cologna C.T., Cardoso Jdos S., Jourdan E., Degueldre M., Upert G.,
RA Gilles N., Uetanabaro A.P., Costa Neto E.M., Thonart P., de Pauw E.,
RA Quinton L.;
RT "Peptidomic comparison and characterization of the major components of the
RT venom of the giant ant Dinoponera quadriceps collected in four different
RT areas of Brazil.";
RL J. Proteomics 94:413-422(2013).
RN [2]
RP REVIEW, AND NOMENCLATURE.
RX PubMed=26805882; DOI=10.3390/toxins8010030;
RA Touchard A., Aili S.R., Fox E.G., Escoubas P., Orivel J., Nicholson G.M.,
RA Dejean A.;
RT "The biochemical toxin arsenal from ant venoms.";
RL Toxins 8:1-28(2016).
CC -!- FUNCTION: M-poneritoxin-Dq4b: this synthetic peptide has antimicrobial
CC activity against Gram-positive bacteria B.amyloliquefacies S499
CC (MIC=0.1 mM), L.monocytogenes 2231 and S.aureus ATCC 29213, against
CC Gram-negative bacteria P.putida BTP1, P.aeruginosa PaO1 and E.coli ATCC
CC 10536, and against the fungi S.cerevisiae, R.mucilaginosa and
CC C.cucumerinum. Not active against the fungi F.oxysporum and B.cinerea.
CC {ECO:0000269|PubMed:24157790}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24157790}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:24157790}.
CC -!- PTM: Occurs in 3 forms, M-PONTX-Dq4b has an amidated Ala-28, U1-PONTX-
CC Dq4d has an amidated Ala-28 and an oxidized Met-20, U1-PONTX-Dq4c has
CC no modifications at either Met-20 or Ala-28.
CC {ECO:0000269|PubMed:24157790}.
CC -!- MASS SPECTROMETRY: Mass=3162.7; Method=Electrospray; Note=M-PONTX-
CC Dq4b.; Evidence={ECO:0000269|PubMed:24157790};
CC -!- MASS SPECTROMETRY: Mass=3163.7; Method=Electrospray; Note=U1-PONTX-
CC Dq4c.; Evidence={ECO:0000269|PubMed:24157790};
CC -!- MASS SPECTROMETRY: Mass=3178.7; Method=Electrospray; Note=U1-PONTX-
CC Dq4d.; Evidence={ECO:0000269|PubMed:24157790};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; C0HJH6; -.
DR Proteomes; UP000515204; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide;
KW Oxidation; Reference proteome; Secreted.
FT PEPTIDE 1..28
FT /note="M-poneritoxin-Dq4b/U1-poneritoxin-Dq4c/U1-
FT poneritoxin-Dq4d"
FT /evidence="ECO:0000269|PubMed:24157790"
FT /id="PRO_0000430022"
FT MOD_RES 20
FT /note="Methionine sulfoxide; in form U1-PONTX-Dq4d"
FT /evidence="ECO:0000269|PubMed:24157790"
FT MOD_RES 28
FT /note="Alanine amide; in form Dq-1362 and U1-PONTX-Dq4d"
FT /evidence="ECO:0000269|PubMed:24157790"
FT UNSURE 2
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24157790"
FT UNSURE 13
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24157790"
FT UNSURE 25
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24157790"
SQ SEQUENCE 28 AA; 3166 MW; 0D76BE0C71072249 CRC64;
GLKDWWNKHK DKIVEVVKEM GKAGLNAA