ID   TX4B_DINAS              Reviewed;          28 AA.
AC   P0CF05;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=M-poneritoxin-Da4b {ECO:0000303|PubMed:26805882};
DE            Short=M-PONTX-Da4b {ECO:0000303|PubMed:26805882};
DE   AltName: Full=Dinoponeratoxin Da-3177 {ECO:0000303|PubMed:19879289};
DE   AltName: Full=Poneratoxin {ECO:0000305};
OS   Dinoponera australis (Giant neotropical hunting ant).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Ponerinae; Ponerini; Dinoponera.
OX   NCBI_TaxID=609289;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, SYNTHESIS, AMIDATION AT ALA-28, AND
RP   SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=19879289; DOI=10.1016/j.toxicon.2009.10.021;
RA   Johnson S.R., Copello J.A., Evans M.S., Suarez A.V.;
RT   "A biochemical characterization of the major peptides from the venom of the
RT   giant Neotropical hunting ant Dinoponera australis.";
RL   Toxicon 55:702-710(2010).
RN   [2]
RP   FUNCTION, AND SYNTHESIS.
RX   PubMed=24157790; DOI=10.1016/j.jprot.2013.10.017;
RA   Cologna C.T., Cardoso Jdos S., Jourdan E., Degueldre M., Upert G.,
RA   Gilles N., Uetanabaro A.P., Costa Neto E.M., Thonart P., de Pauw E.,
RA   Quinton L.;
RT   "Peptidomic comparison and characterization of the major components of the
RT   venom of the giant ant Dinoponera quadriceps collected in four different
RT   areas of Brazil.";
RL   J. Proteomics 94:413-422(2013).
RN   [3]
RP   REVIEW, AND NOMENCLATURE.
RX   PubMed=26805882; DOI=10.3390/toxins8010030;
RA   Touchard A., Aili S.R., Fox E.G., Escoubas P., Orivel J., Nicholson G.M.,
RA   Dejean A.;
RT   "The biochemical toxin arsenal from ant venoms.";
RL   Toxins 8:1-28(2016).
CC   -!- FUNCTION: The synthetic peptide has antimicrobial activity against the
CC       Gram-positive bacteria B.amyloliquefacies S499 (MIC=0.05 mM),
CC       L.monocytogenes 2231 and S.aureus ATCC 29213, against the Gram-negative
CC       bacteria P.putida BTP1, P.aeruginosa PaO1 and E.coli ATCC 10536, and
CC       against the fungi S.cerevisiae, R.mucilaginosa and C.cucumerinum. It is
CC       not active against the fungi F.oxysporum and B.cinerea.
CC       {ECO:0000269|PubMed:24157790}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19879289}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:19879289}.
CC   -!- MASS SPECTROMETRY: Mass=3176.7; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:19879289};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P0CF05; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide;
KW   Secreted.
FT   PEPTIDE         1..28
FT                   /note="M-poneritoxin-Da4b"
FT                   /evidence="ECO:0000269|PubMed:19879289"
FT                   /id="PRO_0000393349"
FT   MOD_RES         28
FT                   /note="Alanine amide"
FT                   /evidence="ECO:0000269|PubMed:19879289"
SQ   SEQUENCE   28 AA;  3180 MW;  0D76AC9C71067FEA CRC64;
     GLKDWWNKHK DKIIDVVKEM GKAGLQAA