TX60B_PHYSE
ID TX60B_PHYSE Reviewed; 488 AA.
AC P58912; A2PYH2;
DT 26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=DELTA-alicitoxin-Pse2b {ECO:0000303|PubMed:22683676};
DE Short=DELTA-ALTX-Pse2b {ECO:0000303|PubMed:22683676};
DE AltName: Full=Toxin PsTX-60B {ECO:0000303|PubMed:17368498};
DE Short=PTX60B;
DE Flags: Precursor;
OS Phyllodiscus semoni (Night anemone).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Nynantheae; Aliciidae; Phyllodiscus.
OX NCBI_TaxID=163701;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=17368498; DOI=10.1016/j.toxicon.2007.01.006;
RA Satoh H., Oshiro N., Iwanaga S., Namikoshi M., Nagai H.;
RT "Characterization of PsTX-60B, a new membrane-attack complex/perforin
RT (MACPF) family toxin, from the venomous sea anemone Phyllodiscus semoni.";
RL Toxicon 49:1208-1210(2007).
RN [2]
RP PROTEIN SEQUENCE OF 22-45, AND FUNCTION.
RC TISSUE=Nematoblast;
RX PubMed=12061771; DOI=10.1016/s0006-291x(02)00547-8;
RA Nagai H., Oshiro N., Takuwa-Kuroda K., Iwanaga S., Nozaki M., Nakajima T.;
RT "Novel proteinaceous toxins from the nematocyst venom of the Okinawan sea
RT anemone Phyllodiscus semoni Kwietniewski.";
RL Biochem. Biophys. Res. Commun. 294:760-763(2002).
RN [3]
RP NOMENCLATURE.
RX PubMed=22683676; DOI=10.1016/j.toxicon.2012.05.020;
RA Oliveira J.S., Fuentes-Silva D., King G.F.;
RT "Development of a rational nomenclature for naming peptide and protein
RT toxins from sea anemones.";
RL Toxicon 60:539-550(2012).
CC -!- FUNCTION: Causes lethal toxicity to the shrimp Palaemon paucidence, and
CC hemolytic activity toward sheep red blood cells.
CC {ECO:0000269|PubMed:12061771}.
CC -!- SUBCELLULAR LOCATION: Secreted. Nematocyst.
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DR EMBL; AB201429; BAF46273.1; -; mRNA.
DR AlphaFoldDB; P58912; -.
DR TCDB; 1.C.39.10.2; the membrane attack complex/perforin (macpf) family.
DR PRIDE; P58912; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR InterPro; IPR020864; MACPF.
DR Pfam; PF01823; MACPF; 1.
DR PROSITE; PS51412; MACPF_2; 1.
PE 1: Evidence at protein level;
KW Cytolysis; Direct protein sequencing; Disulfide bond; Hemolysis;
KW Nematocyst; Secreted; Signal; Toxin.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:12061771"
FT CHAIN 22..488
FT /note="DELTA-alicitoxin-Pse2b"
FT /id="PRO_0000221543"
FT DOMAIN 24..344
FT /note="MACPF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00745"
FT DOMAIN 369..398
FT /note="EGF-like"
FT DISULFID 370..383
FT /evidence="ECO:0000250"
FT DISULFID 377..391
FT /evidence="ECO:0000250"
FT DISULFID 393..398
FT /evidence="ECO:0000250"
SQ SEQUENCE 488 AA; 54461 MW; D9610D169291B3C0 CRC64;
MSKPIIFLLT AFVVLTDLGA TEDTEKVEVK AKPSKTSRGA IGQGFELHKI DLLSKELQGT
GAQVFEELPV EECTTDNKLG TIQKDDTFYS NTESLYNSVA SNSKIEPSLK GPFTLGTSVG
AVTNNIVSEK SEIQGLSLNL KAYSMIHALK QDCINKKPLA KDLVSDFEAL DREVKKPWLK
PSWRKYKVFL EKFGTHIVRE TMSGSSIYQY VFAKSSESFK QRDFKIKACL SLGGPTQVGK
LGISACSDVS KKDVEESSGK EMVKKLVVRG GKSDTRVDLT GELSKNQINK FLKEATTDPS
PIQYTFYPVW TILKARYIGK EHYAKATNLE QFYKGYLNFD CAYEKSEKGL ELQKFELAED
SDPDAPTYVC KLGPEGCHSD DDCESDDLIY CACCGDSCIR YNNTVLLSTG DTKKVAFIND
VDDEFRDHGC GRKGLKCKCK EENKKWGQSW SGDSDADAAL RDINAMMLDQ NRRDQAKRHH
VKKGKSLH
