C6A22_DROME
ID C6A22_DROME Reviewed; 499 AA.
AC Q9V769; Q27592;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Cytochrome P450 6a22;
DE EC=1.14.-.-;
DE AltName: Full=CYPVIA22;
GN Name=Cyp6a22; Synonyms=CYT-P450-RBF6-2; ORFNames=CG10240;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 338-499.
RC STRAIN=91-R;
RX PubMed=8973362; DOI=10.1016/s0378-1119(96)00446-5;
RA Maitra S., Dombrowski S.M., Waters L.C., Ganguly R.;
RT "Three second chromosome-linked clustered Cyp6 genes show differential
RT constitutive and barbital-induced expression in DDT-resistant and
RT susceptible strains of Drosophila melanogaster.";
RL Gene 180:165-171(1996).
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58191.1; -; Genomic_DNA.
DR EMBL; AY102672; AAM27501.1; -; mRNA.
DR EMBL; L46858; AAB41549.1; -; mRNA.
DR PIR; PC4262; PC4262.
DR RefSeq; NP_001286431.1; NM_001299502.1.
DR RefSeq; NP_001286432.1; NM_001299503.1.
DR RefSeq; NP_652075.1; NM_143818.3.
DR AlphaFoldDB; Q9V769; -.
DR SMR; Q9V769; -.
DR BioGRID; 72484; 3.
DR DIP; DIP-17726N; -.
DR IntAct; Q9V769; 3.
DR STRING; 7227.FBpp0086580; -.
DR PaxDb; Q9V769; -.
DR PRIDE; Q9V769; -.
DR DNASU; 49847; -.
DR EnsemblMetazoa; FBtr0087450; FBpp0086580; FBgn0013773.
DR EnsemblMetazoa; FBtr0339983; FBpp0309004; FBgn0013773.
DR EnsemblMetazoa; FBtr0345526; FBpp0311630; FBgn0013773.
DR GeneID; 49847; -.
DR KEGG; dme:Dmel_CG10240; -.
DR CTD; 49847; -.
DR FlyBase; FBgn0013773; Cyp6a22.
DR VEuPathDB; VectorBase:FBgn0013773; -.
DR eggNOG; KOG0158; Eukaryota.
DR GeneTree; ENSGT00940000165972; -.
DR HOGENOM; CLU_001570_5_2_1; -.
DR InParanoid; Q9V769; -.
DR OMA; RTRYSYW; -.
DR OrthoDB; 467733at2759; -.
DR PhylomeDB; Q9V769; -.
DR BioGRID-ORCS; 49847; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 49847; -.
DR PRO; PR:Q9V769; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0013773; Expressed in adult Malpighian tubule (Drosophila) and 18 other tissues.
DR ExpressionAtlas; Q9V769; baseline and differential.
DR Genevisible; Q9V769; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..499
FT /note="Cytochrome P450 6a22"
FT /id="PRO_0000051876"
FT BINDING 439
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 485
FT /note="N -> H (in Ref. 4; AAB41549)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 499 AA; 57278 MW; F12EA2E04CEB3A7B CRC64;
MLDVVALLLI ALAVGFWFVR TRYSYWTRRG IGSEPARFPV GNMEGFRKNK HFIDIVTPIY
EKFKGNGAPF AGFFMMLRPV VLVTDLELAK QILIQDFANF EDRGMYHNER DDPLTGHLFR
IDGPKWRPLR QKMSPTFTSA KMKYMFPTVC EVGEELTQVC GELADNAMCG ILEIGDLMAR
YTSDVIGRCA FGVECNGLRN PEAEFAIMGR RAFSERRHCK LVDGFIESFP EVARFLRMRQ
IHQDITDFYV GIVRETVKQR EEQGIVRSDF MNLLIEMKQR GELTIEEMAA QAFIFFAAGF
DTSASTLGFA LYELAKQPAL QAKLREEIDQ ALRLHNGEFT YDSMQELRYM ELVIAETLRK
YPILPQLTRI SRHLYAAKGD RHFYIEPGQM LLIPVYGIHH DPALYPEPHK FIPERFLADQ
LAQRPTAAWL PFGDGPRNCI GMRFGKMQTT IGLVSLLRNF HFSVCPRTDP KIEFLKSNIL
LCPANGIYLK VQQLSQMSS
