ID   TX90A_PHORI             Reviewed;          55 AA.
AC   P84014;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Omega-ctenitoxin-Pr2a {ECO:0000305};
DE            Short=Omega-CNTX-Pr2a {ECO:0000305};
DE   AltName: Full=Neurotoxin PRTx23C2 {ECO:0000303|Ref.2};
OS   Phoneutria reidyi (Brazilian Amazonian armed spider) (Ctenus reidyi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX   NCBI_TaxID=272752;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=16278100; DOI=10.1016/j.cbpc.2005.09.010;
RA   Richardson M., Pimenta A.M., Bemquerer M.P., Santoro M.M., Beirao P.S.,
RA   Lima M.E., Figueiredo S.G., Bloch C. Jr., Vasconcelos E.A., Campos F.A.,
RA   Gomes P.C., Cordeiro M.N.;
RT   "Comparison of the partial proteomes of the venoms of Brazilian spiders of
RT   the genus Phoneutria.";
RL   Comp. Biochem. Physiol. 142:173-187(2006).
RN   [2]
RP   FUNCTION.
RA   Richardson M., Pimenta A.M.C., Bemquerer M.P., Santoro M.M.,
RA   Figueiredo S.G., Cordeiro M.N.;
RT   "Protein PRTx23C2 from venom of Brazilian Amazonian armed spider Phoneutria
RT   reidyi has sequence similarity with toxin Tx3-6 from the related spider
RT   Phoneutria nigriventer and other spider toxins.";
RL   Submitted (JUN-2004) to UniProtKB.
CC   -!- FUNCTION: Antagonist of L-type calcium channels (Cav1/CACNA1). In vivo,
CC       causes paralysis in posterior limbs, and gradual decrease in movement
CC       and aggression during 24 hours after intracerebroventricular injection
CC       in mice at dose levels of 3 ug per mouse. {ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16278100}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:16278100}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6051.9; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16278100};
CC   -!- SIMILARITY: Belongs to the neurotoxin 09 (Tx3-6) family. {ECO:0000305}.
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DR   AlphaFoldDB; P84014; -.
DR   SMR; P84014; -.
DR   ArachnoServer; AS000239; omega-ctenitoxin-Pr2a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   CHAIN           1..55
FT                   /note="Omega-ctenitoxin-Pr2a"
FT                   /evidence="ECO:0000269|PubMed:16278100"
FT                   /id="PRO_0000087637"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000305"
FT   DISULFID        9..22
FT                   /evidence="ECO:0000305"
FT   DISULFID        15..37
FT                   /evidence="ECO:0000305"
FT   DISULFID        24..35
FT                   /evidence="ECO:0000305"
FT   DISULFID        45..52
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   55 AA;  6062 MW;  E0624FF9C93E5AE0 CRC64;
     ACIPRGEICT DDCECCGCDN ECYCPIGSSL GIFKCSCAHA NKYFCNRKKE KCKKA