ID   TX90E_PHONI             Reviewed;          58 AA.
AC   P84011;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 2.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=U11-ctenitoxin-Pn1b;
DE            Short=U11-CNTX-Pn1b;
DE   AltName: Full=Venom protein PNTx22C3;
OS   Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX   NCBI_TaxID=6918;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Venom {ECO:0000269|PubMed:16278100};
RX   PubMed=16278100; DOI=10.1016/j.cbpc.2005.09.010;
RA   Richardson M., Pimenta A.M., Bemquerer M.P., Santoro M.M., Beirao P.S.,
RA   Lima M.E., Figueiredo S.G., Bloch C. Jr., Vasconcelos E.A., Campos F.A.,
RA   Gomes P.C., Cordeiro M.N.;
RT   "Comparison of the partial proteomes of the venoms of Brazilian spiders of
RT   the genus Phoneutria.";
RL   Comp. Biochem. Physiol. 142:173-187(2006).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND VARIANT HIS-42.
RC   TISSUE=Venom {ECO:0000269|PubMed:16278100};
RA   Richardson M., Pimenta A.M.C., Bemquerer M.P., Santoro M.M.,
RA   Figueiredo S.G., Cordeiro M.N.;
RT   "Protein PNTx22C3 from venom of Brazilian armed spider Phoneutria
RT   nigriventer has sequence similarities with insecticidal toxins from other
RT   spiders.";
RL   Submitted (JUN-2004) to UniProtKB.
CC   -!- FUNCTION: Non-toxic to mice. {ECO:0000269|PubMed:16278100,
CC       ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16278100}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:16278100}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6568.4; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16278100, ECO:0000269|Ref.2};
CC   -!- SIMILARITY: Belongs to the neurotoxin 09 (Tx3-6) family. {ECO:0000305}.
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DR   AlphaFoldDB; P84011; -.
DR   SMR; P84011; -.
DR   ArachnoServer; AS000161; U11-ctenitoxin-Pn1b.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Secreted.
FT   CHAIN           1..58
FT                   /note="U11-ctenitoxin-Pn1b"
FT                   /id="PRO_0000087635"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000305"
FT   DISULFID        9..22
FT                   /evidence="ECO:0000305"
FT   DISULFID        15..40
FT                   /evidence="ECO:0000305"
FT   DISULFID        24..38
FT                   /evidence="ECO:0000305"
FT   DISULFID        48..55
FT                   /evidence="ECO:0000305"
FT   VARIANT         42
FT                   /note="Y -> H"
FT                   /evidence="ECO:0000269|PubMed:16278100, ECO:0000269|Ref.2"
SQ   SEQUENCE   58 AA;  6610 MW;  5A396EB5FBA6A36C CRC64;
     ACLARGETCK DDCECCDCDN QCYCPFDWFG GKWHPVGCSC AYTNKYVCDH KKEKCKKA