ID   TX91O_METSE             Reviewed;          98 AA.
AC   A0A1R3S3A8; A0A1R3S398; C0HK13;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2017, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Peptides MS9.1 {ECO:0000305};
DE   Contains:
DE     RecName: Full=Tau-AnmTX Ms 9a-1 {ECO:0000303|PubMed:28077580};
DE   Contains:
DE     RecName: Full=AnmTx Ms 9a-2 {ECO:0000303|PubMed:28077580};
DE   Flags: Precursor;
OS   Metridium senile (Brown sea anemone) (Frilled sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Nynantheae; Metridiidae; Metridium.
OX   NCBI_TaxID=6116 {ECO:0000312|EMBL:SBO16028.1};
RN   [1] {ECO:0000312|EMBL:SBO16028.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-61, FUNCTION,
RP   SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Tentacle {ECO:0000312|EMBL:SBO16028.1}, and
RC   Venom {ECO:0000303|PubMed:28077580};
RX   PubMed=28077580; DOI=10.1074/jbc.m116.757369;
RA   Logashina Y.A., Mosharova I.V., Korolkova Y.V., Shelukhina I.V.,
RA   Dyachenko I.A., Palikov V.A., Palikova Y.A., Murashev A.N., Kozlov S.A.,
RA   Stensvaag K., Andreev Y.A.;
RT   "Peptide from sea anemone metridium senile affects transient receptor
RT   potential ankyrin-repeat 1 (TRPA1) function and produces analgesic
RT   effect.";
RL   J. Biol. Chem. 292:2992-3004(2017).
CC   -!- FUNCTION: [Tau-AnmTX Ms 9a-1]: Acts as a positive modulator of
CC       mammalian TRPA1, a non-selective cation channel involved in detection
CC       of pain, in vitro yet has an analgesic and anti-inflammatory effect in
CC       vivo. {ECO:0000269|PubMed:28077580}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28077580}.
CC       Nematocyst {ECO:0000269|PubMed:28077580}.
CC   -!- MASS SPECTROMETRY: [Tau-AnmTX Ms 9a-1]: Mass=3654.4; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:28077580};
CC   -!- SIMILARITY: Belongs to the sea anemone structural class 9a family.
CC       {ECO:0000303|PubMed:28077580}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=SBO16029.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; LT577947; SBO16028.1; -; mRNA.
DR   EMBL; LT577948; SBO16029.1; ALT_FRAME; mRNA.
DR   AlphaFoldDB; A0A1R3S3A8; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Nematocyst; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..27
FT                   /evidence="ECO:0000305|PubMed:28077580"
FT                   /id="PRO_0000443941"
FT   PEPTIDE         28..62
FT                   /note="Tau-AnmTX Ms 9a-1"
FT                   /evidence="ECO:0000269|PubMed:28077580"
FT                   /id="PRO_0000443942"
FT   PROPEP          63..64
FT                   /evidence="ECO:0000305|PubMed:28077580"
FT                   /id="PRO_0000443943"
FT   PEPTIDE         65..91
FT                   /note="AnmTx Ms 9a-2"
FT                   /evidence="ECO:0000303|PubMed:28077580"
FT                   /id="PRO_0000443944"
FT   PROPEP          92..98
FT                   /evidence="ECO:0000305|PubMed:28077580"
FT                   /id="PRO_0000443945"
FT   DISULFID        34..46
FT                   /evidence="ECO:0000303|PubMed:28077580"
FT   DISULFID        37..52
FT                   /evidence="ECO:0000303|PubMed:28077580"
FT   CONFLICT        76..80
FT                   /note="KQDSS -> TEDSN (in Ref. 1; SBO16029)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   98 AA;  10208 MW;  4CD6901710EEB5A4 CRC64;
     MKQSLILAVL CLALVFATIE AKPKADPNII VGGCIKCHVK NASGRCVRIV GCGVDKVPDL
     FSDPNIIVGG CSKCHKQDSS GNCVRIAGCG VDPVRDAE