ID   TXA1_ACAGO              Reviewed;          48 AA.
AC   P0DQJ3;
DT   13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2019, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=U1-theraphotoxin-Agm1a {ECO:0000303|PubMed:27436114};
DE            Short=U1-TRTX-Agm1a {ECO:0000303|PubMed:27436114};
OS   Acanthoscurria gomesiana (Tarantula spider) (Phormictopus pheopygus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Acanthoscurria.
OX   NCBI_TaxID=115339;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND
RP   OXIDATION AT MET-44.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=27436114; DOI=10.1016/j.jprot.2016.07.012;
RA   Abreu T.F., Sumitomo B.N., Nishiyama M.Y. Jr., Oliveira U.C., Souza G.H.,
RA   Kitano E.S., Zelanis A., Serrano S.M., Junqueira-de-Azevedo I.,
RA   Silva P.I. Jr., Tashima A.K.;
RT   "Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins
RT   with potential antimicrobial activity.";
RL   J. Proteomics 151:232-242(2017).
CC   -!- FUNCTION: Is toxic to both insects and mammals. Induces reversible
CC       paralysis when injected into S.frugiperda larvae. Reduces both the
CC       amplitude and frequency of responses from muscle (GF-TTM and GF-DLM)
CC       pathways in the D.melanogaster giant fiber circuit, suggesting an
CC       action at the neuromuscular junction, which is mediated by
CC       glutamatergic receptors. In mice, intracranial injection of 30 ug
CC       causes increased urination, myoclonus, hypermotility with circular
CC       movements followed by respiratory and generalized seizures resulting in
CC       death within 25-35 minutes of injection.
CC       {ECO:0000250|UniProtKB:B3EWY4}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27436114}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:27436114}.
CC   -!- MASS SPECTROMETRY: Mass=5457.7; Method=Electrospray; Note=Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:27436114};
CC   -!- MASS SPECTROMETRY: Mass=5473.7; Method=Electrospray; Note=Oxidized
CC       form, monoisotopic mass.; Evidence={ECO:0000269|PubMed:27436114};
CC   -!- SIMILARITY: Belongs to the neurotoxin 12 (Hwtx-2) family. 01 (Ap1a)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DQJ3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012625; Toxin_20.
DR   Pfam; PF08089; Toxin_20; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Neurotoxin; Oxidation; Secreted; Toxin.
FT   CHAIN           1..48
FT                   /note="U1-theraphotoxin-Agm1a"
FT                   /evidence="ECO:0000269|PubMed:27436114"
FT                   /id="PRO_0000448546"
FT   MOD_RES         44
FT                   /note="Methionine sulfoxide; partial"
FT                   /evidence="ECO:0000269|PubMed:27436114"
FT   DISULFID        4..34
FT                   /evidence="ECO:0000250|UniProtKB:B3EWY4"
FT   DISULFID        8..40
FT                   /evidence="ECO:0000250|UniProtKB:B3EWY4"
FT   DISULFID        22..45
FT                   /evidence="ECO:0000250|UniProtKB:B3EWY4"
SQ   SEQUENCE   48 AA;  5468 MW;  29996E3F3BFD3EA4 CRC64;
     IIECFFSCEI EKDGKSKEGK PCKPKGDKDK DKKCSGGWRC KIKMCLKI