ID   TXA1_ACAPA              Reviewed;          98 AA.
AC   B3EWY4;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=U1-theraphotoxin-Ap1a {ECO:0000303|PubMed:23496776};
DE            Short=U1-TRTX-Ap1a {ECO:0000303|PubMed:23496776};
DE   Flags: Precursor;
OS   Acanthoscurria paulensis (Brazilian giant black tarantula spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Acanthoscurria.
OX   NCBI_TaxID=1264770;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-98, FUNCTION,
RP   SUBCELLULAR LOCATION, MASS SPECTROMETRY, TOXIC DOSE, AND DISULFIDE BONDS.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=23496776; DOI=10.1021/bi4000035;
RA   Mourao C.B., Heghinian M.D., Barbosa E.A., Mari F., Bloch C. Jr.,
RA   Restano-Cassulini R., Possani L.D., Schwartz E.F.;
RT   "Characterization of a novel peptide toxin from Acanthoscurria paulensis
RT   spider venom: a distinct cysteine assignment to the HWTX-II family.";
RL   Biochemistry 52:2440-2452(2013).
CC   -!- FUNCTION: Is toxic to both insects and mammals. Induces reversible
CC       paralysis when injected into S.frugiperda larvae. Reduces both the
CC       amplitude and frequency of responses from muscle (GF-TTM and GF-DLM)
CC       pathways in the D.melanogaster giant fiber circuit, suggesting an
CC       action at the neuromuscular junction, which is mediated by
CC       glutamatergic receptors. In mice, intracranial injection of 30 ug
CC       causes increased urination, myoclonus, hypermotility with circular
CC       movements followed by respiratory and generalized seizures resulting in
CC       death within 25-35 minutes of injection. {ECO:0000269|PubMed:23496776}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23496776}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:23496776}.
CC   -!- MASS SPECTROMETRY: Mass=5457.79; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:23496776};
CC   -!- TOXIC DOSE: PD(50) is 13 ug/g in insect larvae (S.frugiperda) (at 8
CC       hours post-injection). {ECO:0000269|PubMed:23496776}.
CC   -!- MISCELLANEOUS: Has no effect on human Nav1.2/SCN2A, Nav1.4/SCN4A,
CC       Nav1.5/SCN5A or Nav1.6/SCN8A voltage-gated potassium channels (at 1
CC       uM). Does not alter the response induced by acetylcholine in a
CC       rhabdomyosarcoma cell preparation (at 1 uM).
CC   -!- SIMILARITY: Belongs to the neurotoxin 12 (Hwtx-2) family. 01 (Ap1a)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; B3EWY4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012625; Toxin_20.
DR   Pfam; PF08089; Toxin_20; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Neurotoxin; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..50
FT                   /evidence="ECO:0000269|PubMed:23496776"
FT                   /id="PRO_0000429250"
FT   PEPTIDE         51..98
FT                   /note="U1-theraphotoxin-Ap1a"
FT                   /evidence="ECO:0000269|PubMed:23496776"
FT                   /id="PRO_0000429251"
FT   REGION          63..84
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        54..84
FT                   /evidence="ECO:0000269|PubMed:23496776"
FT   DISULFID        58..90
FT                   /evidence="ECO:0000269|PubMed:23496776"
FT   DISULFID        72..95
FT                   /evidence="ECO:0000269|PubMed:23496776"
SQ   SEQUENCE   98 AA;  10747 MW;  63CFF2D10613127C CRC64;
     MRSLTLAAVL ACSLLLVFHT SAAEELEVQD GHLMNPGDGD TALATVDDER IIECFFSCEI
     EKDGKSKEGK PCKPKGDKNK DKKCSGGWRC KIKMCLKI