ID   TXA1_LATTR              Reviewed;          88 AA.
AC   P49125;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Alpha-latrotoxin-associated low molecular weight protein {ECO:0000303|PubMed:1509496};
DE            Short=Alpha-latrotoxin-associated LMWP {ECO:0000303|PubMed:1509496};
DE   AltName: Full=Latrodectin-1 {ECO:0000305};
DE            Short=Latrodectin {ECO:0000303|PubMed:7601118};
DE   Flags: Precursor;
OS   Latrodectus tredecimguttatus (Mediterranean black widow spider)
OS   (Latrodectus mactans tredecimguttatus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Araneoidea; Theridiidae; Latrodectus.
OX   NCBI_TaxID=6925;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND PURIFICATION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=1509496; DOI=10.1016/0041-0101(92)90012-t;
RA   Kiyatkin N., Dulubova I., Chekhovskaya I., Lipkin A., Grishin E.;
RT   "Structure of the low molecular weight protein copurified with alpha-
RT   latrotoxin.";
RL   Toxicon 30:771-774(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND PURIFICATION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=7601118; DOI=10.1111/j.1432-1033.1995.tb20566.x;
RA   Pescatori M., Bradbury A., Bouet F., Gargano N., Mastrogiacomo A.,
RA   Grasso A.;
RT   "The cloning of a cDNA encoding a protein (latrodectin) which co-purifies
RT   with the alpha-latrotoxin from the black widow spider Latrodectus
RT   tredecimguttatus (Theridiidae).";
RL   Eur. J. Biochem. 230:322-328(1995).
RN   [3]
RP   PROTEIN SEQUENCE OF 19-88.
RC   TISSUE=Venom;
RX   PubMed=9792186; DOI=10.1016/s0041-0101(98)00162-7;
RA   Grishin E.V.;
RT   "Black widow spider toxins: the present and the future.";
RL   Toxicon 36:1693-1701(1998).
RN   [4]
RP   PROTEIN SEQUENCE OF 19-40.
RC   TISSUE=Venom gland;
RX   PubMed=22001442; DOI=10.1016/j.bcp.2011.09.024;
RA   Graudins A., Little M.J., Pineda S.S., Hains P.G., King G.F., Broady K.W.,
RA   Nicholson G.M.;
RT   "Cloning and activity of a novel alpha-latrotoxin from red-back spider
RT   venom.";
RL   Biochem. Pharmacol. 83:170-183(2012).
RN   [5]
RP   FUNCTION.
RX   PubMed=8051061; DOI=10.1016/s0021-9258(17)32091-4;
RA   Gasparini S., Kiyatkin N., Drevet P., Boulain J.C., Tacnet F., Ripoche P.,
RA   Forest E., Grishin E., Menez A.;
RT   "The low molecular weight protein which co-purifies with alpha-latrotoxin
RT   is structurally related to crustacean hyperglycemic hormones.";
RL   J. Biol. Chem. 269:19803-19809(1994).
RN   [6]
RP   FUNCTION.
RX   PubMed=7570633; DOI=10.1016/0041-0101(94)00166-6;
RA   Volkova T.M., Pluzhnikov K.A., Woll P.G., Grishin E.V.;
RT   "Low molecular weight components from black widow spider venom.";
RL   Toxicon 33:483-489(1995).
CC   -!- FUNCTION: May increase the toxicity of alpha-latrotoxin and/or other
CC       venom components. Is non-toxic to mice and to the cockroach Periplaneta
CC       americana. {ECO:0000269|PubMed:7570633, ECO:0000269|PubMed:8051061}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1509496}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:1509496}.
CC   -!- MISCELLANEOUS: Co-purifies with alpha-latrotoxin-Lt1a.
CC       {ECO:0000269|PubMed:1509496}.
CC   -!- SIMILARITY: Belongs to the arthropod CHH/MIH/GIH/VIH hormone family.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X63116; CAA44830.1; -; mRNA.
DR   EMBL; Z49089; CAA88911.1; -; mRNA.
DR   AlphaFoldDB; P49125; -.
DR   ArachnoServer; AS000288; alpha-Latrotoxin-associated LMWP.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.10.2010.10; -; 1.
DR   InterPro; IPR035957; Crust_neurohorm_sf.
DR   SUPFAM; SSF81778; SSF81778; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000269|PubMed:22001442,
FT                   ECO:0000269|PubMed:9792186"
FT   CHAIN           19..88
FT                   /note="Alpha-latrotoxin-associated low molecular weight
FT                   protein"
FT                   /id="PRO_0000035544"
FT   CONFLICT        31
FT                   /note="Q -> N (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        37
FT                   /note="Missing (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   88 AA;  10006 MW;  A68C8E70C8324BEF CRC64;
     MSKLFFVVFL CLIISVFAIS PADIGCTDIS QADFDEKNNN CIKCGEDGFG EEMVNRCRDK
     CFTDNFYQSC VDLLNKVYEE KDTPPVQE