ID   C7017_ORYSJ             Reviewed;         299 AA.
AC   C7J3A2; A0A0N7KMB0;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Ent-kaurene oxidase-like protein 1 {ECO:0000303|PubMed:15316288};
DE            Short=OsKOL1 {ECO:0000303|PubMed:15316288};
DE   AltName: Full=Cytochrome P450 701A7 {ECO:0000305};
GN   Name=CYP701A7 {ECO:0000305};
GN   Synonyms=KO1 {ECO:0000303|PubMed:15316288},
GN   KOL1 {ECO:0000303|PubMed:15316288};
GN   OrderedLocusNames=Os06g0570600 {ECO:0000312|EMBL:BAH93583.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   TISSUE SPECIFICITY.
RX   PubMed=15316288; DOI=10.1023/b:plan.0000038261.21060.47;
RA   Itoh H., Tatsumi T., Sakamoto T., Otomo K., Toyomasu T., Kitano H.,
RA   Ashikari M., Ichihara S., Matsuoka M.;
RT   "A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin
RT   biosynthesis enzyme, ent-kaurene oxidase.";
RL   Plant Mol. Biol. 54:533-547(2004).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots and panicles.
CC       {ECO:0000269|PubMed:15316288}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
CC       {ECO:0000255|RuleBase:RU000461}.
CC   -!- CAUTION: Could be the product of a pseudogene. In contrast to other
CC       members of the family, it is shorter at the C-terminus and lacks the
CC       heme-binding sites. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP008212; BAH93583.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS98313.1; -; Genomic_DNA.
DR   AlphaFoldDB; C7J3A2; -.
DR   SMR; C7J3A2; -.
DR   STRING; 4530.OS06T0570600-00; -.
DR   PRIDE; C7J3A2; -.
DR   EnsemblPlants; Os06t0570600-00; Os06t0570600-00; Os06g0570600.
DR   Gramene; Os06t0570600-00; Os06t0570600-00; Os06g0570600.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_0_1; -.
DR   InParanoid; C7J3A2; -.
DR   OMA; AKAGWKM; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   GO; GO:0009707; C:chloroplast outer membrane; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0052615; F:ent-kaurene oxidase activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR   GO; GO:0010241; P:ent-kaurene oxidation to kaurenoic acid; IBA:GO_Central.
DR   GO; GO:0009686; P:gibberellin biosynthetic process; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   InterPro; IPR044225; KO_chloroplastic.
DR   PANTHER; PTHR47283; PTHR47283; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   5: Uncertain;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..299
FT                   /note="Ent-kaurene oxidase-like protein 1"
FT                   /id="PRO_0000430735"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   299 AA;  33113 MW;  36E78370F97DA1A6 CRC64;
     MEAFVSGGAG GVGAAAVVGV FVAAAVVGGF VAAVALAERA GVIAPRKRPN APPAVPGLPI
     IGNLHQLKEK KPHQTFTKWA EIYGPIYTIR IGASSVVVLN STEVAKEAMV AKFSSISTRK
     LSKALTVLTR DKSMVATSDY GDFHKMVKRY VMSSTLGTSA QKKFRDTRDM MINNMLSTFH
     KLVKDDPHVP LIFRDVFKDE LFRLSMIQSL GEDVSSVYVD EFGRDISKEE IYNATVTDMM
     MCAIEVDWRD FFSYLSWVPN KSFETRVFTA EARRTAVMRA LIKQQKERIV RGEVTKCIL