ID   TXA8_ANDAU              Reviewed;          80 AA.
AC   Q4LCT0;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Beta-toxin KAaH1;
DE   AltName: Full=Peptide AaF1CA8;
DE   Flags: Precursor;
OS   Androctonus australis (Sahara scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Androctonus.
OX   NCBI_TaxID=6858;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAH03778.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Venom gland {ECO:0000269|PubMed:15963953};
RX   PubMed=15963953; DOI=10.1016/j.bbrc.2005.05.148;
RA   Martin-Eauclaire M.-F., Ceard B., Bosmans F., Rosso J.-P., Tytgat J.,
RA   Bougis P.E.;
RT   "New 'birtoxin analogs' from Androctonus australis venom.";
RL   Biochem. Biophys. Res. Commun. 333:524-530(2005).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 23-80, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Venom {ECO:0000269|PubMed:15656785};
RX   PubMed=15656785; DOI=10.1042/bj20041407;
RA   Srairi-Abid N., Guijarro J.I., Benkhalifa R., Mantegazza M., Cheikh A.,
RA   Ben-Aissa M., Haumont P.-Y., Delepierre M., El Ayeb M.;
RT   "A new type of scorpion Na+-channel-toxin-like polypeptide active on K+
RT   channels.";
RL   Biochem. J. 388:455-464(2005).
CC   -!- FUNCTION: Inhibits the vertebrate potassium channels Kv1.1/KCNA1 and
CC       Kv1.3/KCNA3 in vitro with an IC(50) of 5.3 nM and 50.0 nM respectively.
CC       {ECO:0000269|PubMed:15656785}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15656785}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:15656785, ECO:0000269|PubMed:15963953}.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6652.15; Mass_error=0.67; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:15656785};
CC   -!- MISCELLANEOUS: This toxin is a weak beta toxin because it has no effect
CC       on Nav1.2a/SCN2A voltage-dependent sodium channels when perfused at a
CC       concentration of 100 nM, but when added to the extracellular bath
CC       solution at a concentration of 1 uM it shifts the voltage of activation
CC       toward more negative potentials. Has no effect on the Nav1.5/SCN5A
CC       voltage-dependent sodium channel. Administration to mice produces no
CC       toxic effects (PubMed:15656785). {ECO:0000305|PubMed:15656785}.
CC   -!- SIMILARITY: Belongs to the long (3 C-C) scorpion toxin superfamily.
CC       Sodium/Potassium channel inhibitor family. {ECO:0000305}.
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DR   EMBL; AJ781832; CAH03778.1; -; mRNA.
DR   AlphaFoldDB; Q4LCT0; -.
DR   SMR; Q4LCT0; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0019870; F:potassium channel inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR002061; Scorpion_toxinL/defensin.
DR   Pfam; PF00537; Toxin_3; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51863; LCN_CSAB; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:15656785"
FT   CHAIN           23..80
FT                   /note="Beta-toxin KAaH1"
FT                   /id="PRO_0000228819"
FT   DOMAIN          25..80
FT                   /note="LCN-type CS-alpha/beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        40..63
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        49..68
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        53..70
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
SQ   SEQUENCE   80 AA;  9105 MW;  8095B82721E6F9E1 CRC64;
     MMKLMLFSII VILFSLIGSI HGADVPGNYP LDSSDDTYLC APLGENPFCI KICRKHGVKY
     GYCYAFQCWC EYLEDKNVKI