ID   TXAG1_AGEOR             Reviewed;         114 AA.
AC   Q5Y4U2;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=U10-agatoxin-Ao1a;
DE            Short=U10-AGTX-Ao1a;
DE   AltName: Full=AgorTX_B7b;
DE   Flags: Precursor;
OS   Agelena orientalis (Funnel-web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Agelenidae; Agelena.
OX   NCBI_TaxID=293813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=15688451; DOI=10.1002/prot.20390;
RA   Kozlov S.A., Malyavka A., McCutchen B., Lu A., Schepers E., Herrmann R.,
RA   Grishin E.V.;
RT   "A novel strategy for the identification of toxinlike structures in spider
RT   venom.";
RL   Proteins 59:131-140(2005).
CC   -!- FUNCTION: Inhibits voltage-gated calcium channels (Cav). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 04 (omega-agtx) family. 03 (type
CC       II/III omega-agtx) subfamily. {ECO:0000305}.
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DR   EMBL; AY681343; AAU93684.1; -; mRNA.
DR   AlphaFoldDB; Q5Y4U2; -.
DR   ArachnoServer; AS000070; U10-agatoxin-Ao1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR005853; Omega-agatoxin_II/III_CS.
DR   InterPro; IPR013605; Toxin_34.
DR   Pfam; PF08396; Toxin_34; 1.
DR   PROSITE; PS60023; OMEGA_AGA_II_III; 1.
PE   2: Evidence at transcript level;
KW   Calcium channel impairing toxin; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Presynaptic neurotoxin;
KW   Secreted; Signal; Toxin; Voltage-gated calcium channel impairing toxin.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   PROPEP          16..32
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000093687"
FT   CHAIN           33..112
FT                   /note="U10-agatoxin-Ao1a"
FT                   /id="PRO_5000093688"
FT   REGION          95..114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        34..53
FT                   /evidence="ECO:0000305"
FT   DISULFID        41..59
FT                   /evidence="ECO:0000305"
FT   DISULFID        50..86
FT                   /evidence="ECO:0000305"
FT   DISULFID        52..76
FT                   /evidence="ECO:0000305"
FT   DISULFID        61..74
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   114 AA;  12862 MW;  DA8FF71AF448184F CRC64;
     MCVATCLCTF AYVLAKSDEG ENLISKVEET QRGCIEIGGD CDGYLDKSYC QCCRNNGFCS
     CYKVPEWFGY KVGCKCSVDW NFVGWCRLKQ FCPGGSQNPS LCKDPNPRRR RHGK