ID   TXAG4_AGEOR             Reviewed;          68 AA.
AC   Q5Y4U5;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=U4-agatoxin-Ao1a {ECO:0000305};
DE            Short=U4-AGTX-Ao1a {ECO:0000305};
DE   AltName: Full=Mu-2Aaga_15 {ECO:0000303|PubMed:15688451, ECO:0000312|EMBL:AAU87899.1};
DE   Flags: Precursor;
OS   Agelena orientalis (Funnel-web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Agelenidae; Agelena.
OX   NCBI_TaxID=293813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=15688451; DOI=10.1002/prot.20390;
RA   Kozlov S.A., Malyavka A., McCutchen B., Lu A., Schepers E., Herrmann R.,
RA   Grishin E.V.;
RT   "A novel strategy for the identification of toxinlike structures in spider
RT   venom.";
RL   Proteins 59:131-140(2005).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P86269}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:15688451}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000312|PDB:2N6N}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 33 family. {ECO:0000305}.
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DR   EMBL; AY681339; AAU87899.1; -; mRNA.
DR   PDB; 2N6N; NMR; -; A=37-68.
DR   PDBsum; 2N6N; -.
DR   AlphaFoldDB; Q5Y4U5; -.
DR   BMRB; Q5Y4U5; -.
DR   SMR; Q5Y4U5; -.
DR   ArachnoServer; AS000073; U4-agatoxin-Ao1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   CDD; cd12960; Spider_toxin; 1.
DR   InterPro; IPR004169; Spidertoxin.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Knottin; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   PROPEP          26..36
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000093681"
FT   CHAIN           37..68
FT                   /note="U4-agatoxin-Ao1a"
FT                   /evidence="ECO:0000305|PubMed:15688451"
FT                   /id="PRO_5000093682"
FT   DISULFID        39..52
FT                   /evidence="ECO:0007744|PDB:2N6N"
FT   DISULFID        46..57
FT                   /evidence="ECO:0007744|PDB:2N6N"
FT   DISULFID        51..66
FT                   /evidence="ECO:0007744|PDB:2N6N"
FT   DISULFID        59..64
FT                   /evidence="ECO:0007744|PDB:2N6N"
FT   STRAND          56..58
FT                   /evidence="ECO:0007829|PDB:2N6N"
FT   STRAND          65..67
FT                   /evidence="ECO:0007829|PDB:2N6N"
SQ   SEQUENCE   68 AA;  7446 MW;  000F42E4F04253C5 CRC64;
     MKKSTVIVLS LAAFVLLSVM QFSAAEDIKM EVEEQRGYCA EKGIKCHNIH CCSGLTCKCK
     GSSCVCRK