ID   TXAN1_ACANA             Reviewed;          47 AA.
AC   B3A0P0;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2013, sequence version 3.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Mu-theraphotoxin-An1a {ECO:0000303|PubMed:23651762};
DE            Short=Mu-TRTX-An1a {ECO:0000303|PubMed:23651762};
OS   Acanthoscurria natalensis (Tarantula spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Acanthoscurria.
OX   NCBI_TaxID=1108046;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, DISULFIDE BONDS, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=23651762; DOI=10.1016/j.toxicon.2013.04.013;
RA   Rates B., Prates M.V., Verano-Braga T., da Rocha A.P., Roepstorff P.,
RA   Borges C.L., Lapied B., Murillo L., Pimenta A.M., Biondi I., De Lima M.E.;
RT   "mu-Theraphotoxin-An1a: primary structure determination and assessment of
RT   the pharmacological activity of a promiscuous anti-insect toxin from the
RT   venom of the tarantula Acanthoscurria natalensis (Mygalomorphae,
RT   Theraphosidae).";
RL   Toxicon 70:123-134(2013).
CC   -!- FUNCTION: Is toxic to insects. Reduces amplitude and frequency of
CC       spontaneous firing and inhibits voltage-gated sodium current (Nav) in
CC       the dorsal unpaired median (DUM) neurons of P.americana.
CC       {ECO:0000269|PubMed:23651762}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23651762}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:23651762}.
CC   -!- PTM: Contains 3 disulfide bonds.
CC   -!- MASS SPECTROMETRY: Mass=5370.67; Mass_error=0.01; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:23651762};
CC   -!- SIMILARITY: Belongs to the neurotoxin 12 (Hwtx-2) family. 01 (Ap1a)
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; B3A0P0; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0003254; P:regulation of membrane depolarization; IDA:UniProtKB.
DR   InterPro; IPR012625; Toxin_20.
DR   Pfam; PF08089; Toxin_20; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..47
FT                   /note="Mu-theraphotoxin-An1a"
FT                   /evidence="ECO:0000269|PubMed:23651762"
FT                   /id="PRO_0000420428"
FT   DISULFID        4..34
FT                   /evidence="ECO:0000250|UniProtKB:P49265"
FT   DISULFID        8..39
FT                   /evidence="ECO:0000250|UniProtKB:P49265"
FT   DISULFID        22..44
FT                   /evidence="ECO:0000250|UniProtKB:P49265"
FT   UNSURE          41
FT                   /note="I or L"
FT                   /evidence="ECO:0000305|PubMed:23651762"
FT   UNSURE          45
FT                   /note="I or L"
FT                   /evidence="ECO:0000305|PubMed:23651762"
FT   UNSURE          47
FT                   /note="I or L"
FT                   /evidence="ECO:0000305|PubMed:23651762"
SQ   SEQUENCE   47 AA;  5380 MW;  471B6483C9D9C713 CRC64;
     IIECFFSCEI EKDGKSKEGK PCKPKGDKDK DKKCGGWRCK IKMCIKI