ID   TXDP3_PIRLC             Reviewed;          36 AA.
AC   P83258;
DT   28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Delta-amaurobitoxin-Pl1c;
DE            Short=Delta-AMATX-Pl1c;
DE   AltName: Full=Delta-palutoxin IT3 {ECO:0000303|PubMed:10971590};
DE            Short=Delta-paluIT3 {ECO:0000303|PubMed:10971590};
OS   Pireneitega luctuosa (Tangled nest spider) (Paracoelotes luctuosus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Agelenidae; Pireneitega.
OX   NCBI_TaxID=185217;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=10971590; DOI=10.1046/j.1432-1327.2000.01653.x;
RA   Corzo G., Escoubas P., Stankiewicz M., Pelhate M., Kristensen C.P.,
RA   Nakajima T.;
RT   "Isolation, synthesis and pharmacological characterization of delta-
RT   palutoxins IT, novel insecticidal toxins from the spider Paracoelotes
RT   luctuosus (Amaurobiidae).";
RL   Eur. J. Biochem. 267:5783-5795(2000).
CC   -!- FUNCTION: Binds at site 4 of sodium channels (Nav) and inhibits the
CC       fast inactivation of cockroach channels. This toxin is active only on
CC       insects (By similarity). Has a potent activity against S.litura larvae.
CC       {ECO:0000250, ECO:0000269|PubMed:10971590}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:10971590}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=3926.2; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:10971590};
CC   -!- SIMILARITY: Belongs to the neurotoxin 07 (Beta/delta-agtx) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P83258; -.
DR   SMR; P83258; -.
DR   ArachnoServer; AS000303; delta-Amaurobitoxin-Pl1c.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0035821; P:modulation of process of another organism; IDA:UniProtKB.
DR   InterPro; IPR016328; Beta/delta-agatoxin_fam.
DR   PIRSF; PIRSF001882; Curtatoxin; 1.
DR   PROSITE; PS60015; MU_AGATOXIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   PEPTIDE         1..36
FT                   /note="Delta-amaurobitoxin-Pl1c"
FT                   /id="PRO_0000044963"
FT   DISULFID        3..19
FT                   /evidence="ECO:0000250|UniProtKB:P83257"
FT   DISULFID        10..24
FT                   /evidence="ECO:0000250|UniProtKB:P83257"
FT   DISULFID        18..34
FT                   /evidence="ECO:0000250|UniProtKB:P83257"
FT   DISULFID        26..32
FT                   /evidence="ECO:0000250|UniProtKB:P83257"
SQ   SEQUENCE   36 AA;  3934 MW;  9CDFDAD043A19804 CRC64;
     ADCLNEGDWC ADWSGPSCCG EMWCSCPGFG KCRCKK