ID   C7113_PRUMU             Reviewed;         514 AA.
AC   A0A068Q721;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2014, sequence version 1.
DT   03-AUG-2022, entry version 21.
DE   RecName: Full=Cytochrome P450 71AP13 {ECO:0000303|PubMed:25015725};
DE            EC=1.14.-.- {ECO:0000305};
GN   Name=CYP71AP13 {ECO:0000303|PubMed:25015725};
OS   Prunus mume (Japanese apricot) (Armeniaca mume).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX   NCBI_TaxID=102107;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Nanko; TISSUE=Seedling;
RX   PubMed=25015725; DOI=10.1007/s11103-014-0225-6;
RA   Yamaguchi T., Yamamoto K., Asano Y.;
RT   "Identification and characterization of CYP79D16 and CYP71AN24 catalyzing
RT   the first and second steps in L-phenylalanine-derived cyanogenic glycoside
RT   biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc.";
RL   Plant Mol. Biol. 86:215-223(2014).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000250|UniProtKB:P04798};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in fruit kernel, seedlings, leaves and
CC       stems. {ECO:0000269|PubMed:25015725}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AB920489; BAP15885.1; -; mRNA.
DR   RefSeq; NP_001313441.1; NM_001326512.1.
DR   AlphaFoldDB; A0A068Q721; -.
DR   SMR; A0A068Q721; -.
DR   GeneID; 103337960; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..514
FT                   /note="Cytochrome P450 71AP13"
FT                   /id="PRO_0000449234"
FT   TRANSMEM        20..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         455
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   CARBOHYD        127
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        184
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   514 AA;  58681 MW;  BDFE7BEEC450F520 CRC64;
     MYSVLVQETP MALLQLLKEH SSLFAFSLLI LLLKFIYKDK SRKRRVKLPP SPPKLPVIGN
     LHQLGNKPHL SLRCLAEKYG PIIYLQLGEI PTVVVSSARL AKEVLKTHDL ALSSRPQIFS
     AKHLFYNCTD VVFSPYGAYW RHIRKICILE LLSAKRVQSF SHVREEEVAR LVRRVAEFYP
     GTTNLTKMLG LYANDVLCRV AFGRGFSEGG DYDRHGFQKM LEEYQELLGG FSIGDFFPSM
     EFIHSLTGMK SRLQETFRRF DELFDQMVTD HLSPKREKEE HKDLVDVLLD IQKKESTEMP
     LTMDNVKAII LDMFAAGTDT TFITLDWGMT ELLMNRKVLE RAQAEVRGVV GERRVVLESD
     LPQLDYMKAV IKEIFRLHPP APVLVPRESM EDVTIDGYDI LAKTRIFVNA WAIGRDPESW
     EDPEAFEPER FIGSTIDFKG QDFELIPFGA GRRGCPAVTF GTATIELALA QLLHTFDWEL
     PLDTAAKDLD MTEVFGITMH RIANLIVVAR PRFP