ID   TXHA5_CYRHA             Reviewed;          35 AA.
AC   P60975;
DT   26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2004, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Mu-theraphotoxin-Hhn1a;
DE            Short=Mu-TRTX-Hhn1a;
DE   AltName: Full=Hainantoxin-5 {ECO:0000305};
DE   AltName: Full=Hainantoxin-V {ECO:0000303|PubMed:12727268};
DE            Short=HnTX-V {ECO:0000303|PubMed:12727268};
OS   Cyriopagopus hainanus (Chinese bird spider) (Haplopelma hainanum).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Haplopelma.
OX   NCBI_TaxID=209901;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, DISULFIDE BONDS, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=12727268; DOI=10.1016/s0041-0101(02)00280-5;
RA   Xiao Y.-C., Liang S.-P.;
RT   "Purification and characterization of Hainantoxin-V, a tetrodotoxin-
RT   sensitive sodium channel inhibitor from the venom of the spider
RT   Selenocosmia hainana.";
RL   Toxicon 41:643-650(2003).
CC   -!- FUNCTION: Inhibits selectively tetrodotoxin-sensitive voltage-gated
CC       sodium channels (Nav). Does not act by binding to receptor site 3 to
CC       slow the inactivation kinetics of sodium currents.
CC       {ECO:0000269|PubMed:12727268}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=3972; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12727268};
CC   -!- MISCELLANEOUS: IC(50) is 42.3 nM on rat neurons.
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 22 (Htx-4)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P60975; -.
DR   SMR; P60975; -.
DR   ArachnoServer; AS000341; mu-theraphotoxin-Hhn1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   InterPro; IPR013140; Huwentoxin_CS1.
DR   Pfam; PF07740; Toxin_12; 1.
DR   PROSITE; PS60021; HWTX_1; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Presynaptic neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   PEPTIDE         1..35
FT                   /note="Mu-theraphotoxin-Hhn1a"
FT                   /evidence="ECO:0000269|PubMed:12727268"
FT                   /id="PRO_0000045007"
FT   DISULFID        2..17
FT                   /evidence="ECO:0000250"
FT   DISULFID        9..24
FT                   /evidence="ECO:0000250"
FT   DISULFID        16..31
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   35 AA;  3979 MW;  2588FFD0FC9234C2 CRC64;
     ECLGFGKGCN PSNDQCCKSA NLVCSRKHRW CKYEI