ID   TXHV2_HETVE             Reviewed;          73 AA.
AC   A0A088BP94;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   26-NOV-2014, sequence version 1.
DT   03-AUG-2022, entry version 23.
DE   RecName: Full=Mu-sparatoxin-Hv2 {ECO:0000303|PubMed:29880771};
DE            Short=Mu-SPRTX-Hv2 {ECO:0000303|PubMed:29880771};
DE   AltName: Full=U15-sparatoxin-Hv1a {ECO:0000312|EMBL:AHF45777.1};
DE   Flags: Precursor;
OS   Heteropoda venatoria (Brown huntsman spider) (Aranea venatoria).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Dionycha; Sparassidae; Heteropoda.
OX   NCBI_TaxID=152925;
RN   [1] {ECO:0000312|EMBL:AHF45777.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Chen J., Li Q., He Y., Liang H.;
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 36-44, FUNCTION, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, SYNTHESIS OF 36-44, AND AMIDATION AT PHE-72.
RX   PubMed=29880771; DOI=10.3390/toxins10060233;
RA   Xiao Z., Zhang Y., Zeng J., Liang S., Tang C., Liu Z.;
RT   "Purification and characterization of a novel insecticidal toxin, mu-
RT   sparatoxin-Hv2, from the venom of the spider Heteropoda venatoria.";
RL   Toxins 10:0-0(2018).
CC   -!- FUNCTION: Insecticidal toxin that potently and irreversibly blocks
CC       voltage-gated sodium channels (Nav) in cockroach dorsal unpaired median
CC       (DUM) neurons (IC(50)=833.7 nM) (PubMed:29880771). It does not change
CC       both the steady-state activation and inactivation curves, suggesting it
CC       acts as a pore blocker (possibly at Nav site 1) (PubMed:29880771). Does
CC       not show toxicity when intraperitoneally injected into mouse
CC       (PubMed:29880771). {ECO:0000269|PubMed:29880771}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29880771}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:29880771}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P60590}.
CC   -!- MASS SPECTROMETRY: Mass=4169.51; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:29880771};
CC   -!- TOXIC DOSE: LD(50) is 2.8 nmol/g of body weight, when intraperitoneally
CC       injected into cockroaches. {ECO:0000269|PubMed:29880771}.
CC   -!- MISCELLANEOUS: Hardly affects the DUM voltage-gated potassium channels
CC       (Kv) (15.4% inhibition at 10 uM) and the DUM high-voltage-activated
CC       calcium channels (HVA Cav) (PubMed:29880771). Does not affect or only
CC       very weakly voltage-gated sodium channels Nav1.3/SNC3A (<10% at 15 uM),
CC       Nav1.4//SNC4A (<10% at 15 uM), Nav1.5/SNC5A (no inhibition at 15 uM),
CC       Nav1.7/SNC9A (no inhibition at 15 uM), and Nav1.8/SNC10A (no inhibition
CC       at 15 uM), HVA Cav, and Kv in rat dorsal root ganglion (DRG) neurons
CC       (PubMed:29880771). {ECO:0000269|PubMed:29880771}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family.
CC       {ECO:0000305}.
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DR   EMBL; KC145628; AHF45777.1; -; mRNA.
DR   AlphaFoldDB; A0A088BP94; -.
DR   SMR; A0A088BP94; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Signal; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..35
FT                   /evidence="ECO:0000305|PubMed:29880771"
FT                   /id="PRO_0000451473"
FT   CHAIN           36..72
FT                   /note="Mu-sparatoxin-Hv2"
FT                   /evidence="ECO:0000305|PubMed:29880771"
FT                   /id="PRO_5001836283"
FT   MOD_RES         72
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000305|PubMed:29880771"
FT   DISULFID        39..53
FT                   /evidence="ECO:0000250|UniProtKB:P60590"
FT   DISULFID        46..58
FT                   /evidence="ECO:0000250|UniProtKB:P60590"
FT   DISULFID        52..68
FT                   /evidence="ECO:0000250|UniProtKB:P60590"
SQ   SEQUENCE   73 AA;  8006 MW;  7A00938B520E1F09 CRC64;
     MKFAIVITLL LVAFSAVALA DKSIERAVMD LITARDDDCG KLFADCTSDS DCCENWVCSK
     TGFVKNICKY NFG