TXI11_DIGCA
ID TXI11_DIGCA Reviewed; 58 AA.
AC P55816;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Mu-diguetoxin-Dc1b;
DE Short=Mu-DGTX-Dc1b;
DE AltName: Full=Insecticidal toxin DTX11 {ECO:0000303|PubMed:8541888};
OS Diguetia canities (Desert bush spider) (Segestria canities).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Haplogynae; Pholcoidea; Diguetidae; Diguetia.
OX NCBI_TaxID=38407;
RN [1]
RP PROTEIN SEQUENCE, MASS SPECTROMETRY, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=8541888; DOI=10.1016/0965-1748(95)00029-u;
RA Krapcho K.J., Kral R.M. Jr., Vanwagenen B.C., Eppler K.G., Morgan T.K.;
RT "Characterization and cloning of insecticidal peptides from the primitive
RT weaving spider Diguetia canities.";
RL Insect Biochem. Mol. Biol. 25:991-1000(1995).
CC -!- FUNCTION: Acts by delaying the inactivation of presynaptic voltage-
CC sensitive sodium channels (Nav). Acts against insects and cause a
CC progressive spastic paralysis.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8541888}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:8541888}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=6740; Mass_error=2; Method=FAB;
CC Evidence={ECO:0000269|PubMed:8541888};
CC -!- TOXIC DOSE: PD(50) is 0.71 nmol/g in lepidopteran larvae.
CC {ECO:0000269|PubMed:8541888}.
CC -!- SIMILARITY: Belongs to the neurotoxin 26 (DTX) family. {ECO:0000305}.
CC -!- CAUTION: The measured molecular weight is 27 daltons higher than the
CC expected one suggesting a sequence error. {ECO:0000305}.
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DR AlphaFoldDB; P55816; -.
DR SMR; P55816; -.
DR TCDB; 8.B.30.1.2; the diguetoxin (diguetoxin) family.
DR ArachnoServer; AS000347; mu-diguetoxin-Dc1b.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR035290; Beta/Mu-DGTX-Dc1.
DR Pfam; PF17491; m_DGTX_Dc1a_b_c; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Knottin; Neurotoxin; Presynaptic neurotoxin; Secreted; Toxin;
KW Voltage-gated sodium channel impairing toxin.
FT CHAIN 1..58
FT /note="Mu-diguetoxin-Dc1b"
FT /id="PRO_0000087667"
FT DISULFID 12..26
FT /evidence="ECO:0000250|UniProtKB:P49126"
FT DISULFID 20..40
FT /evidence="ECO:0000250|UniProtKB:P49126"
FT DISULFID 25..54
FT /evidence="ECO:0000250|UniProtKB:P49126"
FT DISULFID 42..52
FT /evidence="ECO:0000250|UniProtKB:P49126"
SQ SEQUENCE 58 AA; 6724 MW; 2480A54CE47B5F05 CRC64;
AKDGDVKGPA GCMKYKSGDC RGKTCCDQQY LWYKWRNLAC RCFTVEVFKK DCWCNDIS