ID   TXI12_DIGCA             Reviewed;          61 AA.
AC   P55817;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   23-FEB-2022, entry version 68.
DE   RecName: Full=Mu-diguetoxin-Dc1c;
DE            Short=Mu-DGTX-Dc1c;
DE   AltName: Full=Insecticidal toxin DTX12 {ECO:0000303|PubMed:8541888};
OS   Diguetia canities (Desert bush spider) (Segestria canities).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Haplogynae; Pholcoidea; Diguetidae; Diguetia.
OX   NCBI_TaxID=38407;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=8541888; DOI=10.1016/0965-1748(95)00029-u;
RA   Krapcho K.J., Kral R.M. Jr., Vanwagenen B.C., Eppler K.G., Morgan T.K.;
RT   "Characterization and cloning of insecticidal peptides from the primitive
RT   weaving spider Diguetia canities.";
RL   Insect Biochem. Mol. Biol. 25:991-1000(1995).
CC   -!- FUNCTION: Acts by delaying the inactivation of presynaptic voltage-
CC       sensitive sodium channels (Nav). Acts against insects and causes a
CC       progressive spastic paralysis.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7080; Mass_error=2; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:8541888};
CC   -!- TOXIC DOSE: PD(50) is 3.19 nmol/g in lepidopteran larvae.
CC       {ECO:0000269|PubMed:8541888}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 26 (DTX) family. {ECO:0000305}.
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DR   TCDB; 8.B.30.1.3; the diguetoxin (diguetoxin) family.
DR   PRIDE; P55817; -.
DR   ArachnoServer; AS000348; mu-diguetoxin-Dc1c.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR035290; Beta/Mu-DGTX-Dc1.
DR   Pfam; PF17491; m_DGTX_Dc1a_b_c; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Presynaptic neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..61
FT                   /note="Mu-diguetoxin-Dc1c"
FT                   /id="PRO_0000087668"
FT   DISULFID        12..25
FT                   /evidence="ECO:0000250|UniProtKB:P49126"
FT   DISULFID        19..39
FT                   /evidence="ECO:0000250|UniProtKB:P49126"
FT   DISULFID        24..53
FT                   /evidence="ECO:0000250|UniProtKB:P49126"
SQ   SEQUENCE   61 AA;  6975 MW;  D27DDBEB1A5C6504 CRC64;
     AKDGDFEGPP GCLKMGELCK GGTCCTKVYK YWKWRKLECL GKNDGWFKKK FICDEPCNPX
     X