ID   TXJ05_LYCSI             Reviewed;          80 AA.
AC   B6DD56;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=U19-lycotoxin-Ls1a {ECO:0000305};
DE            Short=U19-LCTX-Ls1a {ECO:0000305};
DE   AltName: Full=Toxin-like structure LSTX-P5;
DE   Flags: Precursor;
OS   Lycosa singoriensis (Wolf spider) (Aranea singoriensis).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Lycosidae; Lycosa.
OX   NCBI_TaxID=434756;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=19875276; DOI=10.1016/j.zool.2009.04.001;
RA   Zhang Y., Chen J., Tang X., Wang F., Jiang L., Xiong X., Wang M., Rong M.,
RA   Liu Z., Liang S.;
RT   "Transcriptome analysis of the venom glands of the Chinese wolf spider
RT   Lycosa singoriensis.";
RL   Zoology 113:10-18(2010).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19875276}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:19875276}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P30288}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 02 (plectoxin) family. 05 (U19-
CC       lycotoxin) subfamily. {ECO:0000305}.
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DR   EMBL; EU926140; ACI41472.1; -; mRNA.
DR   EMBL; FM864144; CAS03741.1; -; mRNA.
DR   AlphaFoldDB; B6DD56; -.
DR   SMR; B6DD56; -.
DR   ArachnoServer; AS001079; U19-lycotoxin-Ls1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd12960; Spider_toxin; 1.
DR   InterPro; IPR004169; Spidertoxin.
DR   Pfam; PF02819; Toxin_9; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Knottin; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..34
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000401903"
FT   CHAIN           35..80
FT                   /note="U19-lycotoxin-Ls1a"
FT                   /id="PRO_0000401904"
FT   DISULFID        36..50
FT                   /evidence="ECO:0000250|UniProtKB:P30288"
FT   DISULFID        43..55
FT                   /evidence="ECO:0000250|UniProtKB:P30288"
FT   DISULFID        49..66
FT                   /evidence="ECO:0000250|UniProtKB:P30288"
FT   DISULFID        57..64
FT                   /evidence="ECO:0000250|UniProtKB:P30288"
SQ   SEQUENCE   80 AA;  8945 MW;  972D7387ED334219 CRC64;
     MSPKVQALIF IVGLITLLAA HAQEELSDNI ESERGCSGAY KRCSSSQRCC EGRPCVCSAI
     NSNCKCRKTY TELFKEYFEK