ID   TXM14_MACGS             Reviewed;          70 AA.
AC   Q75WG5;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Delta-hexatoxin-Mg1b;
DE            Short=Delta-HXTX-Mg1b;
DE   AltName: Full=Delta-atracotoxin-9;
DE   AltName: Full=Neurotoxin magi-14;
DE   Flags: Precursor;
OS   Macrothele gigas (Japanese funnel web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Macrothelidae; Macrothele.
OX   NCBI_TaxID=223896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=15246762; DOI=10.1016/j.toxicon.2004.05.012;
RA   Satake H., Villegas E., Oshiro N., Terada K., Shinada T., Corzo G.;
RT   "Rapid and efficient identification of cysteine-rich peptides by random
RT   screening of a venom gland cDNA library from the hexathelid spider
RT   Macrothele gigas.";
RL   Toxicon 44:149-156(2004).
CC   -!- FUNCTION: Inhibits tetrodotoxin-sensitive sodium channels (Nav) (By
CC       similarity). Intracranial injection into mice causes strong convulsions
CC       and death. Intrathorax injection into crickets causes paralysis
CC       prolonged for 2 min, followed by recovery. {ECO:0000250,
CC       ECO:0000269|PubMed:15246762}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=5100.00; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15246762};
CC   -!- SIMILARITY: Belongs to the neurotoxin 06 (delta-actx) family.
CC       {ECO:0000305}.
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DR   EMBL; AB121206; BAD13413.1; -; mRNA.
DR   AlphaFoldDB; Q75WG5; -.
DR   SMR; Q75WG5; -.
DR   ArachnoServer; AS000366; delta-hexatoxin-Mg1b.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR008017; Atracotoxin_delta.
DR   Pfam; PF05353; Atracotoxin; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW   Signal; Toxin; Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..26
FT   CHAIN           27..70
FT                   /note="Delta-hexatoxin-Mg1b"
FT                   /id="PRO_0000247527"
FT   DISULFID        27..41
FT                   /evidence="ECO:0000250"
FT   DISULFID        34..46
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..57
FT                   /evidence="ECO:0000250"
FT   DISULFID        42..68
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   70 AA;  8135 MW;  16F8EF6AEB34BC42 CRC64;
     MKILEKALLE NDSAAEEESR NLRTKRCARK RAWCEKTENC CCPMKCIYAW YNGQSSCDHT
     ISTIWTSCPK