ID   TXMG2_MACGS             Reviewed;         121 AA.
AC   P83558; Q75WG9;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 2.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Mu-hexatoxin-Mg1a {ECO:0000305};
DE            Short=Mu-HXTX-Mg1a {ECO:0000305};
DE   AltName: Full=Neurotoxin magi-2 {ECO:0000303|PubMed:12860384, ECO:0000312|EMBL:BAD13409.1};
DE   Flags: Precursor;
OS   Macrothele gigas (Japanese funnel web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Macrothelidae; Macrothele.
OX   NCBI_TaxID=223896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Satake H., Villegas E., Corzo G.;
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 81-120, FUNCTION, SUBCELLULAR LOCATION, TOXIC DOSE,
RP   MASS SPECTROMETRY, AMIDATION AT LYS-120, AND DISULFIDE BONDS.
RC   TISSUE=Venom;
RX   PubMed=12860384; DOI=10.1016/s0014-5793(03)00666-5;
RA   Corzo G., Gilles N., Satake H., Villegas E., Dai L., Nakajima T., Haupt J.;
RT   "Distinct primary structures of the major peptide toxins from the venom of
RT   the spider Macrothele gigas that bind to sites 3 and 4 in the sodium
RT   channel.";
RL   FEBS Lett. 547:43-50(2003).
CC   -!- FUNCTION: Insecticidal neurotoxin. Shows competition for site 3 of
CC       insect voltage-gated sodium channels (Nav). Induces flaccid paralysis
CC       when injected into lepidopteran larvae. Is not toxic to mice when
CC       injected intracranially at 20 pmol/g. {ECO:0000269|PubMed:12860384}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12860384}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:12860384}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=4940.3; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12860384};
CC   -!- TOXIC DOSE: LD(50) is 17.6 nmol/kg to lepidopteran larvae.
CC       {ECO:0000269|PubMed:12860384}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 14 (magi-1) family. 09 (magi-1)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AB121202; BAD13409.1; -; mRNA.
DR   AlphaFoldDB; P83558; -.
DR   ArachnoServer; AS000378; mu-hexatoxin-Mg1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012627; Toxin_22.
DR   Pfam; PF08092; Toxin_22; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW   Signal; Toxin; Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..80
FT                   /evidence="ECO:0000269|PubMed:12860384"
FT                   /id="PRO_0000035586"
FT   CHAIN           81..120
FT                   /note="Mu-hexatoxin-Mg1a"
FT                   /id="PRO_0000035587"
FT   MOD_RES         120
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000269|PubMed:12860384"
FT   DISULFID        81..95
FT                   /evidence="ECO:0000250"
FT   DISULFID        88..102
FT                   /evidence="ECO:0000250"
FT   DISULFID        94..116
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   121 AA;  14188 MW;  407FBC593DA2CFCC CRC64;
     MMTLSPFLLL LIAAVVIGNA SEGEVKNEFE ERLKDEFKDP SRSEVAEVIL LRELEVLEET
     LFGKEMTSDT EENRNSREKR CMGYDIECNE NLPCCKHRKL ECVETSGYWW YKRKYCRPIK
     G