ID   TXMG6_MACGS             Reviewed;          82 AA.
AC   P83562; Q75WG8;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 2.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=U7-hexatoxin-Mg1a;
DE            Short=U7-HXTX-Mg1a;
DE   AltName: Full=Neurotoxin magi-6 {ECO:0000303|PubMed:12860384};
DE   Flags: Precursor;
OS   Macrothele gigas (Japanese funnel web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Macrothelidae; Macrothele.
OX   NCBI_TaxID=223896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Satake H., Villegas E., Corzo G.;
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 45-80, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, TOXIC DOSE, AMIDATION AT ARG-80, MASS SPECTROMETRY, AND
RP   DISULFIDE BONDS.
RC   TISSUE=Venom;
RX   PubMed=12860384; DOI=10.1016/s0014-5793(03)00666-5;
RA   Corzo G., Gilles N., Satake H., Villegas E., Dai L., Nakajima T., Haupt J.;
RT   "Distinct primary structures of the major peptide toxins from the venom of
RT   the spider Macrothele gigas that bind to sites 3 and 4 in the sodium
RT   channel.";
RL   FEBS Lett. 547:43-50(2003).
RN   [3]
RP   TRANSGENIC PROTECTION OF PLANTS.
RX   PubMed=19000914; DOI=10.1016/j.toxicon.2008.10.020;
RA   Hernandez-Campuzano B., Suarez R., Lina L., Hernandez V., Villegas E.,
RA   Corzo G., Iturriaga G.;
RT   "Expression of a spider venom peptide in transgenic tobacco confers insect
RT   resistance.";
RL   Toxicon 53:122-128(2009).
CC   -!- FUNCTION: Induces flaccid paralysis when injected into lepidopteran
CC       larvae. Intracranial injection into mice causes awkwardness of movement
CC       and laboured respiration until death. {ECO:0000269|PubMed:12860384}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12860384,
CC       ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:12860384, ECO:0000305}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=4036.4; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12860384};
CC   -!- TOXIC DOSE: LD(50) is 3.1 nmol/kg in lepidopteran larvae.
CC       {ECO:0000269|PubMed:12860384}.
CC   -!- TOXIC DOSE: LD(50) is 0.74 pmol/g by intracranial injection into mice.
CC       {ECO:0000269|PubMed:12860384}.
CC   -!- MISCELLANEOUS: When expressed in tobacco plants, the toxin induces a
CC       significant resistance to the insect S.frugiperda.
CC   -!- SIMILARITY: Belongs to the rTX family.
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DR   EMBL; AB121203; BAD13410.1; -; mRNA.
DR   AlphaFoldDB; P83562; -.
DR   SMR; P83562; -.
DR   ArachnoServer; AS000386; U7-hexatoxin-Mg1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Knottin; Neurotoxin;
KW   Secreted; Signal; Toxin.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   PROPEP          27..44
FT                   /evidence="ECO:0000269|PubMed:12860384"
FT                   /id="PRO_0000035590"
FT   CHAIN           45..80
FT                   /note="U7-hexatoxin-Mg1a"
FT                   /evidence="ECO:0000269|PubMed:12860384"
FT                   /id="PRO_0000035591"
FT   MOD_RES         80
FT                   /note="Arginine amide"
FT                   /evidence="ECO:0000269|PubMed:12860384"
FT   DISULFID        46..62
FT                   /evidence="ECO:0000305|PubMed:12860384"
FT   DISULFID        51..67
FT                   /evidence="ECO:0000305|PubMed:12860384"
FT   DISULFID        61..77
FT                   /evidence="ECO:0000305|PubMed:12860384"
FT   DISULFID        69..75
FT                   /evidence="ECO:0000305|PubMed:12860384"
SQ   SEQUENCE   82 AA;  9159 MW;  80D33F80A7EC6685 CRC64;
     MRTIVFLIVS ILLLSSAVLM LAEGNAASHE LQEYPIEESL EEQRKCVDGS CDPYSSDAPR
     CCGSQICQCI FFVPCYCKYR GK