ID   TXMG9_MACGS             Reviewed;          80 AA.
AC   Q75WH4;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=U10-hexatoxin-Mg1a;
DE            Short=U10-HXTX-Mg1a;
DE   AltName: Full=Neurotoxin magi-9 {ECO:0000303|PubMed:15246762};
DE   Flags: Precursor;
OS   Macrothele gigas (Japanese funnel web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Macrothelidae; Macrothele.
OX   NCBI_TaxID=223896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MASS SPECTROMETRY, PYROGLUTAMATE
RP   FORMATION AT GLN-44, SUBCELLULAR LOCATION, AND BIOASSAY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=15246762; DOI=10.1016/j.toxicon.2004.05.012;
RA   Satake H., Villegas E., Oshiro N., Terada K., Shinada T., Corzo G.;
RT   "Rapid and efficient identification of cysteine-rich peptides by random
RT   screening of a venom gland cDNA library from the hexathelid spider
RT   Macrothele gigas.";
RL   Toxicon 44:149-156(2004).
RN   [2]
RP   FUNCTION, AND SYNTHESIS OF 44-80.
RX   PubMed=29483648; DOI=10.1038/s41594-018-0033-9;
RA   Correnti C.E., Gewe M.M., Mehlin C., Bandaranayake A.D., Johnsen W.A.,
RA   Rupert P.B., Brusniak M.Y., Clarke M., Burke S.E., De Van Der Schueren W.,
RA   Pilat K., Turnbaugh S.M., May D., Watson A., Chan M.K., Bahl C.D.,
RA   Olson J.M., Strong R.K.;
RT   "Screening, large-scale production and structure-based classification of
RT   cystine-dense peptides.";
RL   Nat. Struct. Mol. Biol. 25:270-278(2018).
CC   -!- FUNCTION: Inhibits Kv11.1/KCNH2/ERG1, Kv1.1/KCNA1, Kv1.2/KCNA2 voltage-
CC       gated potassium channels and Nav1.7/SCN9A voltage-gated sodium channels
CC       (PubMed:29483648). In vivo, no toxicity is observed upon intracranial
CC       injection into mice and intrathorax injection into crickets.
CC       {ECO:0000269|PubMed:15246762, ECO:0000269|PubMed:29483648}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15246762}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:15246762}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=3928.6; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15246762};
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DR   EMBL; AB121197; BAD13404.1; -; mRNA.
DR   AlphaFoldDB; Q75WH4; -.
DR   SMR; Q75WH4; -.
DR   ArachnoServer; AS000389; U10-hexatoxin-Mg1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Disulfide bond; Knottin; Pyrrolidone carboxylic acid; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..43
FT                   /id="PRO_0000285707"
FT   CHAIN           44..80
FT                   /note="U10-hexatoxin-Mg1a"
FT                   /id="PRO_0000285708"
FT   MOD_RES         44
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:15246762"
FT   DISULFID        46..59
FT                   /evidence="ECO:0000305"
FT   DISULFID        53..64
FT                   /evidence="ECO:0000305"
FT   DISULFID        58..75
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   80 AA;  8882 MW;  9077515467977F75 CRC64;
     MKFATFAFTL CVVISLSVLV LADEEEKDFL MNLIQPLKES EERQFCGTNG KPCVNGQCCG
     ALRCVVTYHY ADGVCLKMNP