C71A1_PERAE
ID C71A1_PERAE Reviewed; 502 AA.
AC P24465;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Cytochrome P450 71A1;
DE EC=1.14.-.-;
DE AltName: Full=ARP-2;
DE AltName: Full=CYPLXXIA1;
GN Name=CYP71A1;
OS Persea americana (Avocado).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Magnoliidae; Laurales; Lauraceae; Persea.
OX NCBI_TaxID=3435;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Hass;
RX PubMed=1692626; DOI=10.1073/pnas.87.10.3904;
RA Bozak K.R., Yu H., Sirevag R., Christoffersen R.E.;
RT "Sequence analysis of ripening-related cytochrome P-450 cDNAs from avocado
RT fruit.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:3904-3908(1990).
RN [2]
RP PROTEIN SEQUENCE OF 1-40.
RC TISSUE=Mesocarp;
RX PubMed=16666677; DOI=10.1104/pp.89.4.1141;
RA O'Keefe D.P., Leto K.J.;
RT "Cytochrome P-450 from the mesocarp of avocado (Persea americana).";
RL Plant Physiol. 89:1141-1149(1989).
CC -!- FUNCTION: Involved in the metabolism of compounds associated with the
CC development of flavor in the ripening fruit process, possibly by acting
CC as trans-cinnamic acid 4-hydrolase.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Microsome membrane; Single-pass membrane protein.
CC Endoplasmic reticulum membrane; Single-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Mesocarp.
CC -!- DEVELOPMENTAL STAGE: In ripening fruit.
CC -!- INDUCTION: By ethylene.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; M32885; AAA32913.1; -; mRNA.
DR PIR; A35867; A35867.
DR PIR; T52256; T52256.
DR AlphaFoldDB; P24465; -.
DR SMR; P24465; -.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endoplasmic reticulum; Fruit ripening; Heme;
KW Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..502
FT /note="Cytochrome P450 71A1"
FT /id="PRO_0000052056"
FT TRANSMEM 7..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 443
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 502 AA; 56566 MW; 53427D4F5CD9CD63 CRC64;
MAILVSLLFL AIALTFFLLK LNEKREKKPN LPPSPPNLPI IGNLHQLGNL PHRSLRSLAN
ELGPLILLHL GHIPTLIVST AEIAEEILKT HDLIFASRPS TTAARRIFYD CTDVAFSPYG
EYWRQVRKIC VLELLSIKRV NSYRSIREEE VGLMMERISQ SCSTGEAVNL SELLLLLSSG
TITRVAFGKK YEGEEERKNK FADLATELTT LMGAFFVGDY FPSFAWVDVL TGMDARLKRN
HGELDAFVDH VIDDHLLSRK ANGSDGVEQK DLVDVLLHLQ KDSSLGVHLN RNNLKAVILD
MFSGGTDTTA VTLEWAMAEL IKHPDVMEKA QQEVRRVVGK KAKVEEEDLH QLHYLKLIIK
ETLRLHPVAP LLVPRESTRD VVIRGYHIPA KTRVFINAWA IGRDPKSWEN AEEFLPERFV
NNSVDFKGQD FQLIPFGAGR RGCPGIAFGI SSVEISLANL LYWFNWELPG DLTKEDLDMS
EAVGITVHMK FPLQLVAKRH LS