TXND8_MOUSE
ID TXND8_MOUSE Reviewed; 127 AA.
AC Q69AB2; Q78Y03; Q80YE0; Q9CQ96;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Thioredoxin domain-containing protein 8;
DE AltName: Full=Spermatid-specific thioredoxin-3;
DE Short=Sptrx-3;
DE AltName: Full=Thioredoxin-6;
GN Name=Txndc8; Synonyms=Sptrx3, Trx6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=15181017; DOI=10.1074/jbc.m404192200;
RA Jimenez A., Zu W., Rawe V.Y., Pelto-Huikko M., Flickinger C.J.,
RA Sutovsky P., Gustafsson J.-A., Oko R., Miranda-Vizuete A.;
RT "Spermatocyte/spermatid-specific thioredoxin-3, a novel Golgi apparatus-
RT associated thioredoxin, is a specific marker of aberrant spermatogenesis.";
RL J. Biol. Chem. 279:34971-34982(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=15781233; DOI=10.1016/j.bbrc.2005.02.128;
RA Jimenez A., Prieto-Alamo M.J., Fuentes-Almagro C.A., Jurado J.,
RA Gustafsson J.-A., Pueyo C., Miranda-Vizuete A.;
RT "Absolute mRNA levels and transcriptional regulation of the mouse testis-
RT specific thioredoxins.";
RL Biochem. Biophys. Res. Commun. 330:65-74(2005).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be required for post-translational modifications of
CC proteins required for acrosomal biogenesis. May act by reducing
CC disulfide bonds within the sperm (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15181017}. Golgi
CC apparatus {ECO:0000269|PubMed:15181017}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q69AB2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q69AB2-2; Sequence=VSP_014334;
CC -!- TISSUE SPECIFICITY: Testis-specific. Only expressed during
CC spermiogenesis, prominently in the Golgi apparatus of pachytene
CC spermatocytes and round and elongated spermatids, with a transient
CC localization in the developing acrosome of round spermatids (at protein
CC level). {ECO:0000269|PubMed:15181017}.
CC -!- DEVELOPMENTAL STAGE: First expressed after puberty.
CC {ECO:0000269|PubMed:15781233}.
CC -!- SIMILARITY: Belongs to the thioredoxin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB24702.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY495589; AAS72906.1; -; mRNA.
DR EMBL; AK006683; BAB24702.2; ALT_INIT; mRNA.
DR EMBL; AK015240; BAB29760.2; -; mRNA.
DR EMBL; BC049564; AAH49564.1; -; mRNA.
DR CCDS; CCDS18208.1; -. [Q69AB2-1]
DR RefSeq; NP_080408.2; NM_026132.2. [Q69AB2-1]
DR AlphaFoldDB; Q69AB2; -.
DR SMR; Q69AB2; -.
DR STRING; 10090.ENSMUSP00000099961; -.
DR iPTMnet; Q69AB2; -.
DR PhosphoSitePlus; Q69AB2; -.
DR PaxDb; Q69AB2; -.
DR PRIDE; Q69AB2; -.
DR ProteomicsDB; 298398; -. [Q69AB2-1]
DR ProteomicsDB; 298399; -. [Q69AB2-2]
DR Antibodypedia; 48474; 33 antibodies from 8 providers.
DR DNASU; 67402; -.
DR Ensembl; ENSMUST00000102897; ENSMUSP00000099961; ENSMUSG00000038709. [Q69AB2-1]
DR Ensembl; ENSMUST00000239406; ENSMUSP00000159291; ENSMUSG00000038709. [Q69AB2-2]
DR GeneID; 67402; -.
DR KEGG; mmu:67402; -.
DR CTD; 255220; -.
DR MGI; MGI:1914652; Txndc8.
DR VEuPathDB; HostDB:ENSMUSG00000038709; -.
DR eggNOG; KOG0907; Eukaryota.
DR GeneTree; ENSGT00940000162445; -.
DR HOGENOM; CLU_090389_14_6_1; -.
DR InParanoid; Q69AB2; -.
DR OMA; FHAMSLK; -.
DR TreeFam; TF321403; -.
DR BioGRID-ORCS; 67402; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Txndc8; mouse.
DR PRO; PR:Q69AB2; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q69AB2; protein.
DR Bgee; ENSMUSG00000038709; Expressed in spermatid and 13 other tissues.
DR ExpressionAtlas; Q69AB2; baseline and differential.
DR Genevisible; Q69AB2; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IBA:GO_Central.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR InterPro; IPR017937; Thioredoxin_CS.
DR InterPro; IPR013766; Thioredoxin_domain.
DR Pfam; PF00085; Thioredoxin; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS00194; THIOREDOXIN_1; 1.
DR PROSITE; PS51352; THIOREDOXIN_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW Disulfide bond; Golgi apparatus; Redox-active center; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..127
FT /note="Thioredoxin domain-containing protein 8"
FT /id="PRO_0000120163"
FT DOMAIN 2..127
FT /note="Thioredoxin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT DISULFID 32..35
FT /note="Redox-active"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT VAR_SEQ 1
FT /note="M -> MMNGTATVILHLNQQERVSSKSQMLIM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_014334"
FT CONFLICT Q69AB2-2:2
FT /note="M -> R (in Ref. 2; BAB24702)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 127 AA; 14517 MW; ADE894C04EE104EE CRC64;
MVKRIKNMSE LKELFSDAGN KLVVVEFSAK WCGPCKTIAP VFQAMSLKYQ NVTFAQVDVD
SSKELAEHCD ITMLPTFQMF KYTQKVTPFS RLKRVLCCLR SGPKSKMIFE CHGADAKQLE
KKIQELM
