ID   TXP5_BRASM              Reviewed;          34 AA.
AC   P49266;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=U2-theraphotoxin-Bs1a;
DE            Short=U2-TRTX-Bs1a;
DE   AltName: Full=BsTX5;
DE   AltName: Full=TXP5;
DE   AltName: Full=Venom protein 5;
OS   Brachypelma smithi (Mexican red knee tarantula) (Eurypelma smithi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Brachypelma.
OX   NCBI_TaxID=54074;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND DISULFIDE BOND.
RC   TISSUE=Venom;
RX   PubMed=7801344; DOI=10.1016/0041-0101(94)90392-1;
RA   Kaiser I.I., Griffin P.R., Aird S.D., Hudiburg S., Shabanowitz J.,
RA   Francis B., John T.R., Hunt D.F., Odell G.V.;
RT   "Primary structures of two proteins from the venom of the Mexican red knee
RT   tarantula (Brachypelma smithii).";
RL   Toxicon 32:1083-1093(1994).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=3960.5; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:7801344};
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 03 (BsTX5)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P49266; -.
DR   SMR; P49266; -.
DR   ArachnoServer; AS000406; U2-theraphotoxin-Bs1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   InterPro; IPR013140; Huwentoxin_CS1.
DR   Pfam; PF07740; Toxin_12; 1.
DR   PROSITE; PS60021; HWTX_1; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Neurotoxin; Secreted;
KW   Toxin.
FT   PEPTIDE         1..34
FT                   /note="U2-theraphotoxin-Bs1a"
FT                   /id="PRO_0000044999"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000250"
FT   DISULFID        9..21
FT                   /evidence="ECO:0000269|PubMed:7801344"
FT   DISULFID        15..28
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   34 AA;  3966 MW;  86B3688088C4C686 CRC64;
     SCVDFQTKCK KDSDCCGKLE CSSRWKWCVY PSPF