ID   TXP7_APOSC              Reviewed;          32 AA.
AC   P49271;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=U3-cyrtautoxin-As1a;
DE            Short=U3-CUTX-As1a;
DE   AltName: Full=Aptotoxin VII;
DE   AltName: Full=Aptotoxin-7;
DE   AltName: Full=Paralytic peptide VII;
DE            Short=PP VII;
OS   Apomastus schlingeri (Trap-door spider) (Aptostichus schlingeri).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Euctenizidae; Apomastus.
OX   NCBI_TaxID=12944;
RN   [1]
RP   PROTEIN SEQUENCE, AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=1440641; DOI=10.1016/0041-0101(92)90049-b;
RA   Skinner W.S., Dennis P.A., Li J.P., Quistad G.B.;
RT   "Identification of insecticidal peptides from venom of the trap-door
RT   spider, Aptostichus schlingeri (Ctenizidae).";
RL   Toxicon 30:1043-1050(1992).
RN   [2]
RP   STRUCTURE BY NMR, AND DISULFIDE BONDS.
RA   Kobayashi K., Kim J.-I., Sato K., Kohno T.;
RT   "Three-dimensional solution structure of aptotoxin-VII, from the venom of a
RT   trap-door spider.";
RL   Submitted (DEC-2005) to the PDB data bank.
CC   -!- FUNCTION: Is both paralytic and lethal, when injected into lepidopteran
CC       larvae. Is a slower acting toxin, being lethal at 24 hours, but not
CC       paralytic at 1 hour post-injection.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC   -!- TOXIC DOSE: LD(50) is 1.40 mg/kg by subcutaneous injection.
CC       {ECO:0000269|PubMed:1440641}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 14 (magi-1) family.
CC   -!- SIMILARITY: To aptotoxin III. {ECO:0000305}.
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DR   PIR; B44007; B44007.
DR   PDB; 1WQB; NMR; -; A=1-32.
DR   PDBsum; 1WQB; -.
DR   AlphaFoldDB; P49271; -.
DR   SMR; P49271; -.
DR   ArachnoServer; AS000408; U3-cyrtautoxin-As1a.
DR   EvolutionaryTrace; P49271; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Knottin;
KW   Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..32
FT                   /note="U3-cyrtautoxin-As1a"
FT                   /id="PRO_0000044987"
FT   DISULFID        4..19
FT                   /evidence="ECO:0000269|Ref.2"
FT   DISULFID        11..24
FT                   /evidence="ECO:0000269|Ref.2"
FT   DISULFID        18..29
FT                   /evidence="ECO:0000269|Ref.2"
FT   STRAND          13..16
FT                   /evidence="ECO:0007829|PDB:1WQB"
FT   STRAND          23..30
FT                   /evidence="ECO:0007829|PDB:1WQB"
SQ   SEQUENCE   32 AA;  3537 MW;  2AFB15230F06BCF6 CRC64;
     WLGCARVKEA CGPWEWPCCS GLKCDGSECH PQ