TXPA_BACSU
ID TXPA_BACSU Reviewed; 59 AA.
AC P54373;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Toxin TxpA {ECO:0000303|PubMed:16166525};
GN Name=txpA {ECO:0000303|PubMed:16166525}; Synonyms=yqcR, yqdB;
GN OrderedLocusNames=BSU26050;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=7704261; DOI=10.1099/13500872-141-2-323;
RA Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.;
RT "Complete nucleotide sequence of a skin element excised by DNA
RT rearrangement during sporulation in Bacillus subtilis.";
RL Microbiology 141:323-327(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION, INDUCTION, AND OVEREXPRESSION.
RC STRAIN=168 / PY79;
RX PubMed=16166525; DOI=10.1128/jb.187.19.6641-6650.2005;
RA Silvaggi J.M., Perkins J.B., Losick R.;
RT "Small untranslated RNA antitoxin in Bacillus subtilis.";
RL J. Bacteriol. 187:6641-6650(2005).
CC -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system
CC (PubMed:16166525). Overexpression of txpA causes cell lysis; the TxpA
CC protein has been suggested to act on the cell membrane or might
CC possibly block cell wall synthesis (PubMed:16166525). Overexpression in
CC E.coli is not toxic (PubMed:16166525). {ECO:0000269|PubMed:16166525,
CC ECO:0000305|PubMed:16166525}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: The antisense RNA ratA acts as an antitoxin by annealing to
CC the mRNA of txpA and causing its degradation, protecting the cell from
CC TxpA by blocking the production of the toxin. A type I toxin-antitoxin
CC (TA) system, where expression of the proteinaceous toxin is controlled
CC by an antisense sRNA. {ECO:0000269|PubMed:16166525}.
CC -!- MISCELLANEOUS: Encoded in a 48-kb, phage-like element known as skin.
CC {ECO:0000305|PubMed:7704261}.
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DR EMBL; D32216; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; D84432; BAA12409.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14546.1; -; Genomic_DNA.
DR PIR; E69950; E69950.
DR RefSeq; NP_390482.1; NC_000964.3.
DR RefSeq; WP_004398662.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P54373; -.
DR STRING; 224308.BSU26050; -.
DR PaxDb; P54373; -.
DR EnsemblBacteria; CAB14546; CAB14546; BSU_26050.
DR GeneID; 8303122; -.
DR KEGG; bsu:BSU26050; -.
DR PATRIC; fig|224308.179.peg.2831; -.
DR BioCyc; BSUB:BSU26050-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Toxin-antitoxin system;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..59
FT /note="Toxin TxpA"
FT /id="PRO_0000049781"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 59 AA; 6739 MW; 8A7EEF81ACD97719 CRC64;
MSTYESLMVM IGFANLIGGI MTWVISLLTL LFMLRKKDTH PIYITVKEKC LHEDPPIKG
