ID   TXPM1_PHYCF             Reviewed;          32 AA.
AC   C0HJB1;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 1.
DT   25-MAY-2022, entry version 16.
DE   RecName: Full=Pi-phymatoxin-Pcf1a {ECO:0000305};
DE            Short=Pi-PMTX-Pcf1a {ECO:0000305};
DE   AltName: Full=Pi-PMTX-Pcr1a {ECO:0000303|PubMed:23764262};
DE   AltName: Full=Toxin PhcrTx1 {ECO:0000303|PubMed:23764262};
OS   Phymanthus crucifer (Red beaded anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Phymanthidae; Phymanthus.
OX   NCBI_TaxID=1291152;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RX   PubMed=23764262; DOI=10.1016/j.peptides.2013.06.003;
RA   Rodriguez A.A., Salceda E., Garateix A.G., Zaharenko A.J., Peigneur S.,
RA   Lopez O., Pons T., Richardson M., Diaz M., Hernandez Y., Standker L.,
RA   Tytgat J., Soto E.;
RT   "A novel sea anemone peptide that inhibits acid-sensing ion channels.";
RL   Peptides 53:3-12(2014).
CC   -!- FUNCTION: Reversibly inhibits acid-sensing ion channels (ASIC) in rat
CC       dorsal root ganglia neurons (IC(50)=100 nM). Reversibly inhibits
CC       voltage-gated potassium channels (Kv) in rat DRG neurons (IC(50)=3.5
CC       uM). {ECO:0000269|PubMed:23764262}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Nematocyst {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=3477; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:23764262};
CC   -!- MISCELLANEOUS: Has no effect on voltage-gated sodium channels. Not
CC       toxic to crabs when administered by in vivo injection (2mg/kg crab).
CC       {ECO:0000269|PubMed:23764262}.
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DR   AlphaFoldDB; C0HJB1; -.
DR   SMR; C0HJB1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Nematocyst; Neurotoxin; Proton-gated sodium channel impairing toxin;
KW   Secreted; Toxin.
FT   PEPTIDE         1..32
FT                   /note="Pi-phymatoxin-Pcf1a"
FT                   /evidence="ECO:0000269|PubMed:23764262"
FT                   /id="PRO_0000423820"
FT   DISULFID        1..15
FT                   /evidence="ECO:0000250|UniProtKB:A0A6I8WFP9"
FT   DISULFID        8..20
FT                   /evidence="ECO:0000250|UniProtKB:A0A6I8WFP9"
FT   DISULFID        14..28
FT                   /evidence="ECO:0000250|UniProtKB:A0A6I8WFP9"
SQ   SEQUENCE   32 AA;  3482 MW;  75B6161D20A592DA CRC64;
     CASQGQKCKT KSDCCNGMWC AGTRGHTCYK PK