TXPM2_PHYCF
ID TXPM2_PHYCF Reviewed; 46 AA.
AC C0HK75;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 1.
DT 25-MAY-2022, entry version 9.
DE RecName: Full=Toxin PhcrTx2 {ECO:0000303|PubMed:29414882};
OS Phymanthus crucifer (Red beaded anemone).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Phymanthidae; Phymanthus.
OX NCBI_TaxID=1291152 {ECO:0000303|PubMed:29414882};
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND TOXIC DOSE.
RX PubMed=29414882; DOI=10.3390/toxins10020072;
RA Rodriguez A.A., Garateix A., Salceda E., Peigneur S., Zaharenko A.J.,
RA Pons T., Santos Y., Arreguin R., Staendker L., Forssmann W.G., Tytgat J.,
RA Vega R., Soto E.;
RT "PhcrTx2, a New Crab-Paralyzing Peptide Toxin from the Sea Anemone
RT Phymanthus crucifer.";
RL Toxins 10:0-0(2018).
CC -!- FUNCTION: Paralyzes but does not kill U.thayeri crabs. Partially and
CC reversibly inhibits glutamate-evoked peak currents (IC(50)=4.7 uM) but
CC not voltage-gated potassium channel currents in cultured isolated
CC neurons from the land snail H.aspersa. Weakly inhibits voltage-gated
CC potassium peak currents (IC(50)=6.4 uM) and steady-state currents
CC (IC(50)=8.2 uM) in rat dorsal root ganglion (DRG) neurons. Weakly
CC inhibits voltage-gated sodium currents in rat DRG neurons (IC(50)=0.9
CC uM). {ECO:0000269|PubMed:29414882}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:29414882}.
CC Nematocyst {ECO:0000305|PubMed:29414882}.
CC -!- MASS SPECTROMETRY: Mass=5296.8; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:29414882};
CC -!- TOXIC DOSE: PD(50) is 707 ug/kg in U.thayeri crabs.
CC {ECO:0000269|PubMed:29414882}.
CC -!- MISCELLANEOUS: Has no inhibitory effect on voltage-gated ion channels
CC Kv1.1/KCNA1, Kv1.2/KCNA2, Kv1.3/KCNA3, Kv1.4/KCNA4, Kv1.6/KCNA6,
CC Kv2.1/KCNB1, Kv3.1/KCNC1, Kv4.2/KCND2, Kv10.1/KCNH1/EAG1, Shaker IR
CC (with the inactivation domain removed), Nav1.4/SCN4A, Nav1.5/SCN5A,
CC Nav1.6/SCN8A or D.melanogaster Nav1/para.
CC {ECO:0000269|PubMed:29414882}.
CC -!- SIMILARITY: Belongs to the sea anemone type 3 (BDS) potassium channel
CC toxin family. {ECO:0000305}.
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DR AlphaFoldDB; C0HK75; -.
DR SMR; C0HK75; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 2.20.20.10; -; 1.
DR InterPro; IPR012414; BDS_K_chnl_tox.
DR InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR Pfam; PF07936; Defensin_4; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Nematocyst; Potassium channel impairing toxin; Secreted; Toxin;
KW Voltage-gated potassium channel impairing toxin;
KW Voltage-gated sodium channel impairing toxin.
FT CHAIN 1..46
FT /note="Toxin PhcrTx2"
FT /evidence="ECO:0000269|PubMed:29414882"
FT /id="PRO_0000443732"
FT DISULFID 4..40
FT /evidence="ECO:0000250|UniProtKB:P11494"
FT DISULFID 6..32
FT /evidence="ECO:0000250|UniProtKB:P11494"
FT DISULFID 22..41
FT /evidence="ECO:0000250|UniProtKB:P11494"
SQ SEQUENCE 46 AA; 5303 MW; 4565F71AFB68962E CRC64;
ALPCRCEGKT EYGDKWIFHG GCPNDYGYND RCFMKPGSVC CYPKYE
