ID   TXPR1_ALOMR             Reviewed;          35 AA.
AC   P86269;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Purotoxin-1 {ECO:0000303|PubMed:20437566};
DE            Short=PT1 {ECO:0000303|PubMed:20437566};
OS   Alopecosa marikovskyi (Wolf spider) (Lycosa kazakhstanicus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Lycosidae; Alopecosa.
OX   NCBI_TaxID=2066572;
RN   [1]
RP   PROTEIN SEQUENCE, STRUCTURE BY NMR, FUNCTION, SUBCELLULAR LOCATION, AND
RP   DISULFIDE BONDS.
RC   TISSUE=Venom;
RX   PubMed=20437566; DOI=10.1002/ana.21949;
RA   Grishin E.V., Savchenko G.A., Vassilevski A.A., Korolkova Y.V.,
RA   Boychuk Y.A., Viatchenko-Karpinski V.Y., Nadezhdin K.D., Arseniev A.S.,
RA   Pluzhnikov K.A., Kulyk V.B., Voitenko N.V., Krishtal O.O.;
RT   "Novel peptide from spider venom inhibits P2X3 receptors and inflammatory
RT   pain.";
RL   Ann. Neurol. 67:680-683(2010).
RN   [2]
RP   FUNCTION.
RX   PubMed=22842000; DOI=10.1016/j.bbamem.2012.07.016;
RA   Kabanova N.V., Vassilevski A.A., Rogachevskaja O.A., Bystrova M.F.,
RA   Korolkova Y.V., Pluzhnikov K.A., Romanov R.A., Grishin E.V.,
RA   Kolesnikov S.S.;
RT   "Modulation of P2X3 receptors by spider toxins.";
RL   Biochim. Biophys. Acta 1818:2868-2875(2012).
CC   -!- FUNCTION: Inhibits P2RX3 receptors. Has an analgesic effect in rat.
CC       Enhances the high-affinity desensitization of P2RX3 receptors. At 50
CC       nM, decreases the IC(50) for ambient ATP from 46.5 nM to 12.7 nM in
CC       mouse P2RX3. {ECO:0000269|PubMed:20437566,
CC       ECO:0000269|PubMed:22842000}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20437566}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:20437566}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000269|PubMed:20437566}.
CC   -!- MISCELLANEOUS: Does not affect mouse P2RX2 or P2RX2/P2RX3 receptors.
CC       {ECO:0000269|PubMed:22842000}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 33 family. {ECO:0000305}.
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DR   PDB; 2KGU; NMR; -; A=1-35.
DR   PDBsum; 2KGU; -.
DR   AlphaFoldDB; P86269; -.
DR   SMR; P86269; -.
DR   PRIDE; P86269; -.
DR   EvolutionaryTrace; P86269; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019855; F:calcium channel inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd12960; Spider_toxin; 1.
DR   InterPro; IPR004169; Spidertoxin.
PE   1: Evidence at protein level;
KW   3D-structure; Calcium channel impairing toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Knottin; Neurotoxin; Secreted;
KW   Toxin.
FT   PEPTIDE         1..35
FT                   /note="Purotoxin-1"
FT                   /evidence="ECO:0000269|PubMed:20437566"
FT                   /id="PRO_0000395427"
FT   DISULFID        3..16
FT                   /evidence="ECO:0000269|PubMed:20437566,
FT                   ECO:0000312|PDB:2KGU"
FT   DISULFID        10..21
FT                   /evidence="ECO:0000269|PubMed:20437566,
FT                   ECO:0000312|PDB:2KGU"
FT   DISULFID        15..32
FT                   /evidence="ECO:0000269|PubMed:20437566,
FT                   ECO:0000312|PDB:2KGU"
FT   DISULFID        23..30
FT                   /evidence="ECO:0000269|PubMed:20437566,
FT                   ECO:0000312|PDB:2KGU"
FT   STRAND          11..13
FT                   /evidence="ECO:0007829|PDB:2KGU"
FT   STRAND          20..22
FT                   /evidence="ECO:0007829|PDB:2KGU"
FT   STRAND          25..27
FT                   /evidence="ECO:0007829|PDB:2KGU"
FT   STRAND          31..33
FT                   /evidence="ECO:0007829|PDB:2KGU"
SQ   SEQUENCE   35 AA;  3845 MW;  C861E5E17FB26D4B CRC64;
     GYCAEKGIRC DDIHCCTGLK CKCNASGYNC VCRKK