ID   TXR1_MACRV              Reviewed;          43 AA.
AC   P61233;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2004, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=U5-hexatoxin-Mr1a;
DE            Short=U5-HXTX-Mr1a;
DE   AltName: Full=Raventoxin I;
DE   AltName: Full=Raventoxin-1;
OS   Macrothele raveni (Funnel-web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Macrothelidae; Macrothele.
OX   NCBI_TaxID=269627;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, CHARACTERIZATION, MASS SPECTROMETRY, AND TOXIC
RP   DOSE.
RC   TISSUE=Venom;
RX   PubMed=12727269; DOI=10.1016/s0041-0101(02)00361-6;
RA   Zeng X.-Z., Xiao Q.-B., Liang S.-P.;
RT   "Purification and characterization of raventoxin-I and raventoxin-III, two
RT   neurotoxic peptides from the venom of the spider Macrothele raveni.";
RL   Toxicon 41:651-656(2003).
CC   -!- FUNCTION: This toxin blocks the neuromuscular transmission, and also
CC       acts on muscle. It exerts an effect of first exciting and then
CC       inhibiting the contraction of muscle. This toxin is active only against
CC       mammals. {ECO:0000269|PubMed:12727269}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- PTM: Contains 4 disulfide bonds.
CC   -!- MASS SPECTROMETRY: Mass=4841.11; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12727269};
CC   -!- TOXIC DOSE: LD(50) is 0.772 mg/kg by intraabdominal injection into
CC       mice. {ECO:0000269|PubMed:12727269}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 35 family.
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DR   AlphaFoldDB; P61233; -.
DR   SMR; P61233; -.
DR   ArachnoServer; AS000416; U5-hexatoxin-Mr1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Neurotoxin; Secreted;
KW   Toxin.
FT   CHAIN           1..43
FT                   /note="U5-hexatoxin-Mr1a"
FT                   /id="PRO_0000087680"
FT   DISULFID        1..16
FT                   /evidence="ECO:0000305"
FT   DISULFID        8..21
FT                   /evidence="ECO:0000305"
FT   DISULFID        15..36
FT                   /evidence="ECO:0000305"
FT   DISULFID        17..43
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   43 AA;  4849 MW;  99DCF87DDFB88441 CRC64;
     CGTNRAWCRN AKDHCCCGYS CVKPIWASKP EDDGYCWKKF GGC