TXTPA_DANRE
ID TXTPA_DANRE Reviewed; 359 AA.
AC Q6PGY3;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Tricarboxylate transport protein A, mitochondrial {ECO:0000250|UniProtKB:P53007};
DE AltName: Full=Citrate transport protein A {ECO:0000250|UniProtKB:P53007};
DE AltName: Full=Solute carrier family 25 member 1a {ECO:0000312|ZFIN:ZDB-GENE-040426-1172};
DE Flags: Precursor;
GN Name=slc25a1a {ECO:0000312|ZFIN:ZDB-GENE-040426-1172};
GN ORFNames=zgc:63578 {ECO:0000312|EMBL:AAH56787.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26870663; DOI=10.3233/jnd-140021;
RA Chaouch A., Porcelli V., Cox D., Edvardson S., Scarcia P., De Grassi A.,
RA Pierri C.L., Cossins J., Laval S.H., Griffin H., Mueller J.S.,
RA Evangelista T., Toepf A., Abicht A., Huebner A., von der Hagen M.,
RA Bushby K., Straub V., Horvath R., Elpeleg O., Palace J., Senderek J.,
RA Beeson D., Palmieri L., Lochmueller H.;
RT "Mutations in the mitochondrial citrate carrier SLC25A1 are associated with
RT impaired neuromuscular transmission.";
RL J. Neuromuscul. Dis. 1:75-90(2014).
CC -!- FUNCTION: Citrate transporter that mediates the exchange of
CC mitochondrial citrate for cytosolic malate (By similarity). Required
CC for proper neuromuscular junction formation (PubMed:26870663).
CC {ECO:0000250|UniProtKB:P53007, ECO:0000269|PubMed:26870663}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q8JZU2}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Morpholino-injected embryos display altered tail
CC morphology, impaired swimming and defective touch-evoked escape
CC responses. Neuromuscular junction development is abnormal and motor
CC axon terminals show short and erratic outgrowth toward the muscle fiber
CC with no evidence of complete synapse formation. In addition, knockdown
CC embryos often show edema of the hindbrain, heart, yolk sac and tail.
CC {ECO:0000269|PubMed:26870663}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000255, ECO:0000255|RuleBase:RU000488}.
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DR EMBL; CR926463; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC056787; AAH56787.1; -; mRNA.
DR RefSeq; NP_956901.1; NM_200607.1.
DR AlphaFoldDB; Q6PGY3; -.
DR SMR; Q6PGY3; -.
DR STRING; 7955.ENSDARP00000074163; -.
DR PaxDb; Q6PGY3; -.
DR Ensembl; ENSDART00000079711; ENSDARP00000074163; ENSDARG00000057110.
DR GeneID; 393579; -.
DR KEGG; dre:393579; -.
DR CTD; 393579; -.
DR ZFIN; ZDB-GENE-040426-1172; slc25a1a.
DR eggNOG; KOG0756; Eukaryota.
DR GeneTree; ENSGT00940000165695; -.
DR HOGENOM; CLU_015166_5_1_1; -.
DR InParanoid; Q6PGY3; -.
DR OMA; VRFLFFD; -.
DR OrthoDB; 1523221at2759; -.
DR PhylomeDB; Q6PGY3; -.
DR TreeFam; TF105786; -.
DR PRO; PR:Q6PGY3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 10.
DR Bgee; ENSDARG00000057110; Expressed in mature ovarian follicle and 26 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0071913; F:citrate secondary active transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006843; P:mitochondrial citrate transmembrane transport; IBA:GO_Central.
DR GO; GO:0007528; P:neuromuscular junction development; IMP:UniProtKB.
DR GO; GO:0010821; P:regulation of mitochondrion organization; IMP:ZFIN.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..47
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 48..359
FT /note="Tricarboxylate transport protein A, mitochondrial"
FT /id="PRO_0000447226"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 71..159
FT /note="Solcar 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 170..256
FT /note="Solcar 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 266..351
FT /note="Solcar 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
SQ SEQUENCE 359 AA; 39412 MW; 052BF3F80261C282 CRC64;
MSSLYKPFCV SEGPCKDAIL RRGCAPLQHP AAPTRIPSPG GGSTRTFTPI PNIYPGQRTL
AAAAIGGRKI THPWKAIFAG GIAGGIEICI TFPTEYVKTQ LQLDERANPP RYRGIGDCVK
LTVQYHGLRG LYRGLSSLLY GSIPKSAVRF GTFEVLSNPM RDATGRLDNK ASLLCGLGAG
IAEAVLVVCP METVKVKFIH DQCSLRPRYR GFFHGVREII RDQGVRGTYQ GLTATLLKQG
SNQAIRFYVM NLLRNWYKGD DPARDMHPLV TAMFGATAGA ASVFGNTPLD VVKTRMQGLE
AHRYKSTMDC AFQILKNEGP LAFYKGTVPR LGRVCLDVAI VFVLYEEVVK LLNNVWRTD
