ID   TXTP_BOVIN              Reviewed;         311 AA.
AC   P79110;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Tricarboxylate transport protein, mitochondrial;
DE   AltName: Full=Citrate transport protein;
DE            Short=CTP;
DE   AltName: Full=Solute carrier family 25 member 1;
DE   AltName: Full=Tricarboxylate carrier protein;
DE   Flags: Precursor;
GN   Name=SLC25A1; Synonyms=SLC20A3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8865808; DOI=10.1016/0005-2736(96)00115-0;
RA   Iacobazzi V., de Palma A., Palmieri F.;
RT   "Cloning and sequencing of the bovine cDNA encoding the mitochondrial
RT   tricarboxylate carrier protein.";
RL   Biochim. Biophys. Acta 1284:9-12(1996).
CC   -!- FUNCTION: Citrate transporter that mediates the exchange of
CC       mitochondrial citrate for cytosolic malate. Also able to mediate the
CC       exchange of citrate for isocitrate, phosphoenolpyruvate, cis- but not
CC       trans-aconitate and to a lesser extend maleate and succinate. Important
CC       for the bioenergetics of hepatic cells as it provides a carbon source
CC       for fatty acid and sterol biosyntheses, and NAD(+) for the glycolytic
CC       pathway. Required for proper neuromuscular junction formation.
CC       {ECO:0000250|UniProtKB:P53007}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q8JZU2}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
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DR   EMBL; X97773; CAA66375.1; -; mRNA.
DR   RefSeq; NP_777081.1; NM_174656.2.
DR   AlphaFoldDB; P79110; -.
DR   SMR; P79110; -.
DR   STRING; 9913.ENSBTAP00000011242; -.
DR   PaxDb; P79110; -.
DR   PeptideAtlas; P79110; -.
DR   PRIDE; P79110; -.
DR   GeneID; 282476; -.
DR   CTD; 6576; -.
DR   eggNOG; KOG0756; Eukaryota.
DR   InParanoid; P79110; -.
DR   OrthoDB; 1523221at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0071913; F:citrate secondary active transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006843; P:mitochondrial citrate transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.50.40.10; -; 1.
DR   InterPro; IPR002067; Mit_carrier.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   Pfam; PF00153; Mito_carr; 3.
DR   PRINTS; PR00926; MITOCARRIER.
DR   SUPFAM; SSF103506; SSF103506; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW   Reference proteome; Repeat; Transit peptide; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..13
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250|UniProtKB:P32089"
FT   CHAIN           14..311
FT                   /note="Tricarboxylate transport protein, mitochondrial"
FT                   /id="PRO_0000019261"
FT   TRANSMEM        29..46
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..105
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..143
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..202
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..241
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        278..297
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          23..111
FT                   /note="Solcar 1"
FT   REPEAT          122..208
FT                   /note="Solcar 2"
FT   REPEAT          218..303
FT                   /note="Solcar 3"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         156
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P53007"
SQ   SEQUENCE   311 AA;  33876 MW;  75E010BC872F59BE CRC64;
     MAAARAPRAL TSASPGSGKA KLTHPGKAIL AGGLAGGIEI CITFPTEYVK TQLQLDERSH
     PPRYRGIGDC VRQTVRSHGL LGLYRGLSSL LYGSIPKAAV RFGTFEFLSN HMRDAQGRLD
     STRGLLCGLG AGVPEAVVVV CPMETIKVKF IHDQTSASPK YRGFFHGVRE IVREQGLKGT
     YQGLTATVLK QGSNQGIRFF VMTSLRNWYR GDNPNKPMNP LITGVFGAIA GAASVFGNTP
     LDVIKTRMQG LEAHKYRNTL DCGLQILRNE GLKAFYKGTV PRLGRVCLDV AIVFIIYDEV
     VKLLNKVWKA D