TXTP_RAT
ID TXTP_RAT Reviewed; 311 AA.
AC P32089; Q498T8;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Tricarboxylate transport protein, mitochondrial;
DE AltName: Full=Citrate transport protein;
DE Short=CTP;
DE AltName: Full=Solute carrier family 25 member 1;
DE AltName: Full=Tricarboxylate carrier protein;
DE Flags: Precursor;
GN Name=Slc25a1; Synonyms=Slc20a3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=8514800; DOI=10.1016/s0021-9258(19)38701-0;
RA Kaplan R.S., Mayor J.A., Wood D.O.;
RT "The mitochondrial tricarboxylate transport protein. cDNA cloning, primary
RT structure, and comparison with other mitochondrial transport proteins.";
RL J. Biol. Chem. 268:13682-13690(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Citrate transporter that mediates the exchange of
CC mitochondrial citrate for cytosolic malate. Also able to mediate the
CC exchange of citrate for isocitrate, phosphoenolpyruvate, cis- but not
CC trans-aconitate and to a lesser extend maleate and succinate. Important
CC for the bioenergetics of hepatic cells as it provides a carbon source
CC for fatty acid and sterol biosyntheses, and NAD(+) for the glycolytic
CC pathway. Required for proper neuromuscular junction formation.
CC {ECO:0000250|UniProtKB:P53007}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q8JZU2}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; L12016; AAA18899.1; -; mRNA.
DR EMBL; BC100077; AAI00078.1; -; mRNA.
DR PIR; A46595; A46595.
DR RefSeq; NP_059003.1; NM_017307.3.
DR AlphaFoldDB; P32089; -.
DR SMR; P32089; -.
DR BioGRID; 248354; 2.
DR IntAct; P32089; 3.
DR STRING; 10116.ENSRNOP00000000306; -.
DR TCDB; 2.A.29.7.1; the mitochondrial carrier (mc) family.
DR CarbonylDB; P32089; -.
DR iPTMnet; P32089; -.
DR PhosphoSitePlus; P32089; -.
DR jPOST; P32089; -.
DR PaxDb; P32089; -.
DR PRIDE; P32089; -.
DR GeneID; 29743; -.
DR KEGG; rno:29743; -.
DR CTD; 6576; -.
DR RGD; 3703; Slc25a1.
DR VEuPathDB; HostDB:ENSRNOG00000038001; -.
DR eggNOG; KOG0756; Eukaryota.
DR HOGENOM; CLU_015166_5_1_1; -.
DR InParanoid; P32089; -.
DR OMA; CPAFIVG; -.
DR OrthoDB; 1523221at2759; -.
DR PhylomeDB; P32089; -.
DR TreeFam; TF105786; -.
DR Reactome; R-RNO-70263; Gluconeogenesis.
DR Reactome; R-RNO-75105; Fatty acyl-CoA biosynthesis.
DR PRO; PR:P32089; -.
DR Proteomes; UP000002494; Chromosome 11.
DR Bgee; ENSRNOG00000038001; Expressed in ovary and 19 other tissues.
DR Genevisible; P32089; RN.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; ISO:RGD.
DR GO; GO:0071913; F:citrate secondary active transmembrane transporter activity; ISO:RGD.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; TAS:RGD.
DR GO; GO:0006843; P:mitochondrial citrate transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Phosphoprotein; Reference proteome; Repeat;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..13
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:8514800"
FT CHAIN 14..311
FT /note="Tricarboxylate transport protein, mitochondrial"
FT /id="PRO_0000019263"
FT TRANSMEM 29..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..105
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..143
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..202
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..241
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..297
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 23..111
FT /note="Solcar 1"
FT REPEAT 122..208
FT /note="Solcar 2"
FT REPEAT 218..303
FT /note="Solcar 3"
FT MOD_RES 156
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53007"
SQ SEQUENCE 311 AA; 33835 MW; C7B5B8D82311F6D6 CRC64;
MAAPRAPRAL TAAAPGSGKA KLTHPGKAIL AGGLAGGIEI CITFPTEYVK TQLQLDERAN
PPRYRGIGDC VRQTVRSHGV LGLYRGLSSL LYGSIPKAAV RFGMFEFLSN HMRDAQGRLD
SRRGLLCGLG AGVAEAVVVV CPMETVKVKF IHDQTSSNPK YRGFFHGVRE IVREQGLKGT
YQGLTATVLK QGSNQAIRFF VMTSLRNWYQ GDNPNKPMNP LITGVFGAVA GAASVFGNTP
LDVIKTRMQG LEAHKYRNTL DCGVQILKNE GPKAFYKGTV PRLGRVCLDV AIVFVIYDEV
VKLLNKVWKT D
