ID   TXU2_HETVE              Reviewed;          30 AA.
AC   P61792;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2004, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Kappa-sparatoxin-Hv1d {ECO:0000305};
DE            Short=Kappa-SPRTX-Hv1d {ECO:0000305};
DE   AltName: Full=Toxin AU2 {ECO:0000303|Ref.1};
OS   Heteropoda venatoria (Brown huntsman spider) (Aranea venatoria).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Dionycha; Sparassidae; Heteropoda.
OX   NCBI_TaxID=152925;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RA   Kelbaugh P.R., Saccomano N.A., Volkmann R.A.;
RT   "Calcium channel blocking polypeptides from Heteropoda venatoria.";
RL   Patent number US5627154, 06-MAY-1997.
CC   -!- FUNCTION: Inhibitor of voltage-gated potassium channels of the Kv4/KCND
CC       family (By similarity). Blocks calcium channels (Cav). {ECO:0000250,
CC       ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P58426}.
CC   -!- MASS SPECTROMETRY: Mass=3316.97; Method=Electrospray;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 19 (HpTX2)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P61792; -.
DR   SMR; P61792; -.
DR   ArachnoServer; AS000419; kappa-sparatoxin-Hv1d.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin;
KW   Potassium channel impairing toxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   PEPTIDE         1..30
FT                   /note="Kappa-sparatoxin-Hv1d"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000044554"
FT   DISULFID        3..17
FT                   /evidence="ECO:0000250|UniProtKB:P58426"
FT   DISULFID        10..22
FT                   /evidence="ECO:0000250|UniProtKB:P58426"
FT   DISULFID        16..26
FT                   /evidence="ECO:0000250|UniProtKB:P58426"
SQ   SEQUENCE   30 AA;  3325 MW;  E6F495AAE695CFCF CRC64;
     DDCGGLFSGC DSNADCCEGY VCRLWCKYKL