C71AC_ARATH
ID C71AC_ARATH Reviewed; 497 AA.
AC O49340; F4INX7; Q8LPP9;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Cytochrome P450 71A12;
DE EC=1.14.-.- {ECO:0000305};
GN Name=CYP71A12; OrderedLocusNames=At2g30750; ORFNames=T11J7.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26352477; DOI=10.1038/nature14907;
RA Rajniak J., Barco B., Clay N.K., Sattely E.S.;
RT "A new cyanogenic metabolite in Arabidopsis required for inducible pathogen
RT defence.";
RL Nature 525:376-379(2015).
CC -!- FUNCTION: Converts indole-3-acetaldoxime to indole cyanohydrin.
CC Involved in the biosynthetic pathway to 4-hydroxyindole-3-carbonyl
CC nitrile (4-OH-ICN), a cyanogenic metabolite required for inducible
CC pathogen defense. {ECO:0000269|PubMed:26352477}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: 90% decrease of all indole-3-carbonyl nitrile
CC (ICN) derivatives and increased susceptibility to virulent Pseudomonas
CC syringae. {ECO:0000269|PubMed:26352477}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM19850.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAN46800.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC002340; AAC02746.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08435.2; -; Genomic_DNA.
DR EMBL; AY094484; AAM19850.1; ALT_INIT; mRNA.
DR EMBL; BT001046; AAN46800.1; ALT_INIT; mRNA.
DR PIR; C84712; C84712.
DR RefSeq; NP_180633.3; NM_128628.4.
DR AlphaFoldDB; O49340; -.
DR SMR; O49340; -.
DR BioGRID; 2975; 2.
DR STRING; 3702.AT2G30750.1; -.
DR PaxDb; O49340; -.
DR PRIDE; O49340; -.
DR ProteomicsDB; 222826; -.
DR EnsemblPlants; AT2G30750.1; AT2G30750.1; AT2G30750.
DR GeneID; 817626; -.
DR Gramene; AT2G30750.1; AT2G30750.1; AT2G30750.
DR KEGG; ath:AT2G30750; -.
DR Araport; AT2G30750; -.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_0_1; -.
DR InParanoid; O49340; -.
DR OMA; MEMILMV; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; O49340; -.
DR PRO; PR:O49340; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O49340; baseline and differential.
DR Genevisible; O49340; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..497
FT /note="Cytochrome P450 71A12"
FT /id="PRO_0000052063"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 439
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 497 AA; 56152 MW; BE6AC1A28A737C81 CRC64;
MEMILMVSLC LTTLITLFLL KQFLKRTANK VNLPPSPWRL PLIGNLHQLS LHPHRSLHSL
SLRYGPLMLL HFGRVPILVV SSGEAAQEVL KTHDLKFANR PRSKAVHGLM NGGRDVVFGP
YGEYWRQMKS VCILNLLTNK MVASFEKIRE EELNEMIKKL EKASSSSSSE NLSELFVTLP
SDVTSRIALG RKHSEDETAR DLKKRVRQIM ELLGEFPIGD YVPALAWIDR INGFNARIKE
VSQGFSDLMD KVVQEHLEAG NHKEDFVDIL LSIESEKSIG FQAQRDDIKF MILDMFIGGT
STSSTLLEWI MTELIRNPNV MKKLQDEIRS TIRPHGSYIK EKDVENMKYL KAVIKEVFRV
HPPLPLILPR LLSEDVKVKG YNIAAGTEVI INAWAIQRDP AIWGPDAEEF KPERHLDSTL
DYHGKDLNFI PFGSGRRICP GINLALGLVE VTVANLVGRF DWRAEAGPNG DQPDLTEAFG
LDVCRKFPLI AFPSSVI
