ID   TXVE_DABSI              Reviewed;         109 AA.
AC   P0DL42;
DT   27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT   27-MAY-2015, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Snake venom vascular endothelial growth factor toxin VR-1' {ECO:0000303|PubMed:15992764};
DE            Short=svVEGF;
DE   AltName: Full=VEGF-F {ECO:0000303|PubMed:15992764};
OS   Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Viperinae; Daboia.
OX   NCBI_TaxID=343250;
RN   [1]
RP   PROTEIN SEQUENCE, PYROGLUTAMATE FORMATION AT GLU-1, SUBCELLULAR LOCATION,
RP   AND NOMENCLATURE.
RC   TISSUE=Venom;
RX   PubMed=15992764; DOI=10.1016/j.abb.2005.05.020;
RA   Tokunaga Y., Yamazaki Y., Morita T.;
RT   "Specific distribution of VEGF-F in Viperinae snake venoms: isolation and
RT   characterization of a VGEF-F from the venom of Daboia russelli siamensis.";
RL   Arch. Biochem. Biophys. 439:241-247(2005).
RN   [2]
RP   PROTEIN SEQUENCE OF 45-55 AND 97-106, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=19457351; DOI=10.1016/j.jprot.2009.01.006;
RA   Risch M., Georgieva D., von Bergen M., Jehmlich N., Genov N., Arni R.K.,
RA   Betzel C.;
RT   "Snake venomics of the Siamese Russell's viper (Daboia russelli siamensis)
RT   -- relation to pharmacological activities.";
RL   J. Proteomics 72:256-269(2009).
CC   -!- FUNCTION: Stimulates vascular endothelial cell proliferation
CC       (EC(50)=200 pM) in a similar manner than VR-1 and vammin
CC       (PubMed:15992764). This angiogenesis probably occurs through VEGF
CC       receptor (KDR/VEGFR-2) signaling. Also induces drastic hypotension
CC       which is mediated by nitric oxide (NO) (which is produced by VEGF-
CC       activated endothelium NO synthase). Also induces capillary permeability
CC       (By similarity). {ECO:0000250|UniProtKB:P67861,
CC       ECO:0000269|PubMed:15992764}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000250|UniProtKB:P67861}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15992764}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MISCELLANEOUS: The new subtype VEGF-F is proposed to group snake venom
CC       VEGFs that specifically bind to KDR/VEGFR-2.
CC       {ECO:0000303|PubMed:15992764}.
CC   -!- SIMILARITY: Belongs to the PDGF/VEGF growth factor family. Snake venom
CC       VEGF subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DL42; -.
DR   SMR; P0DL42; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00135; PDGF; 1.
DR   Gene3D; 2.10.90.10; -; 1.
DR   InterPro; IPR029034; Cystine-knot_cytokine.
DR   InterPro; IPR023581; PD_growth_factor_CS.
DR   InterPro; IPR000072; PDGF/VEGF_dom.
DR   Pfam; PF00341; PDGF; 1.
DR   SMART; SM00141; PDGF; 1.
DR   SUPFAM; SSF57501; SSF57501; 1.
DR   PROSITE; PS00249; PDGF_1; 1.
DR   PROSITE; PS50278; PDGF_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Growth factor;
KW   Pyrrolidone carboxylic acid; Secreted; Toxin.
FT   CHAIN           1..109
FT                   /note="Snake venom vascular endothelial growth factor toxin
FT                   VR-1'"
FT                   /evidence="ECO:0000250|UniProtKB:P67861"
FT                   /id="PRO_0000433035"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid (Glu)"
FT                   /evidence="ECO:0000269|PubMed:15992764"
FT   DISULFID        14..56
FT                   /evidence="ECO:0000250|UniProtKB:P67861"
FT   DISULFID        39
FT                   /note="Interchain (with C-48)"
FT                   /evidence="ECO:0000250|UniProtKB:P67861"
FT   DISULFID        45..91
FT                   /evidence="ECO:0000250|UniProtKB:P67861"
FT   DISULFID        48
FT                   /note="Interchain (with C-39)"
FT                   /evidence="ECO:0000250|UniProtKB:P67861"
FT   DISULFID        49..93
FT                   /evidence="ECO:0000250|UniProtKB:P67861"
SQ   SEQUENCE   109 AA;  12554 MW;  7765E67F8CF929E3 CRC64;
     EVRPFLDVYQ RSACQTRETL VSILQEHPDE ISDIFRPSCV AVLRCSGCCT DESMKCTPVG
     KHTADIQIMR MNPRTHSSKM EVMKFMEHTA CECRPRWKQG EPEGPKEPR