C71AG_ARATH
ID C71AG_ARATH Reviewed; 497 AA.
AC Q9FH66; Q0V7W0;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Cytochrome P450 71A16;
DE EC=1.14.-.-;
DE AltName: Full=Marneral oxidase {ECO:0000303|PubMed:28734978};
GN Name=CYP71A16;
GN Synonyms=MRNO {ECO:0000303|PubMed:23570231},
GN MRO {ECO:0000303|PubMed:28734978}; OrderedLocusNames=At5g42590;
GN ORFNames=K16E1.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=23570231; DOI=10.1021/ja401535g;
RA Castillo D.A., Kolesnikova M.D., Matsuda S.P.;
RT "An effective strategy for exploring unknown metabolic pathways by genome
RT mining.";
RL J. Am. Chem. Soc. 135:5885-5894(2013).
RN [5]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=28734978; DOI=10.1016/j.bbapap.2017.07.008;
RA Kranz-Finger S., Mahmoud O., Ricklefs E., Ditz N., Bakkes P.J.,
RA Urlacher V.B.;
RT "Insights into the functional properties of the marneral oxidase CYP71A16
RT from Arabidopsis thaliana.";
RL Biochim. Biophys. Acta 1866:2-10(2018).
CC -!- FUNCTION: Possesses triterpene oxidizing activity. Catalyzes the C23
CC hydroxylation of marneral to form 23-hydroxymarneral. Catalyzes the C23
CC hydroxylation of marnerol to form 23-hydroxymarnerol.
CC {ECO:0000269|PubMed:23570231, ECO:0000269|PubMed:28734978}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=142 uM for marnerol {ECO:0000269|PubMed:28734978};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AB022210; BAB09330.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94832.1; -; Genomic_DNA.
DR EMBL; BT026460; ABH04567.1; -; mRNA.
DR RefSeq; NP_199073.1; NM_123623.5.
DR AlphaFoldDB; Q9FH66; -.
DR SMR; Q9FH66; -.
DR BioGRID; 19516; 1.
DR STRING; 3702.AT5G42590.1; -.
DR iPTMnet; Q9FH66; -.
DR PaxDb; Q9FH66; -.
DR PRIDE; Q9FH66; -.
DR ProteomicsDB; 240260; -.
DR EnsemblPlants; AT5G42590.1; AT5G42590.1; AT5G42590.
DR GeneID; 834266; -.
DR Gramene; AT5G42590.1; AT5G42590.1; AT5G42590.
DR KEGG; ath:AT5G42590; -.
DR Araport; AT5G42590; -.
DR TAIR; locus:2152701; AT5G42590.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_0_1; -.
DR InParanoid; Q9FH66; -.
DR OMA; QRDKATW; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; Q9FH66; -.
DR PRO; PR:Q9FH66; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FH66; baseline and differential.
DR Genevisible; Q9FH66; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IDA:UniProtKB.
DR GO; GO:0006722; P:triterpenoid metabolic process; IDA:UniProtKB.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..497
FT /note="Cytochrome P450 71A16"
FT /id="PRO_0000052067"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 439
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 497 AA; 55628 MW; 9F712BB0ECE1A166 CRC64;
MEMMILISLC LTTFLTILLF FKSLLKRPNS NLPPSPWRLP VIGNLHQLSL HPHRALSSLS
ARHGPLMLLR FGRVPVLIVS SADVAHDVMK THDLKFANRP ITKSAHKISN GGRDLVFAPY
GEYWRNVKSL CTIHLLSNKM VQSSEKRREE EITLLMETLE EASLSSSSVN LSKLITNMVS
DIMGKVVLGK KYSGEEGTID VKTITKSFLD AVGLSPVGEY IPSLAWIGKI TGSDGKLEKI
TKQFGDFIEK VLQEHEDTTA DKETPDFVDM LLTIQRDETA QCQLDKSDLK VIIFEMFLGS
TTTTSAVIEW AMTRLMRNPE CLKKLQDEIR SVSKMNSYVS GKEVENMNYL KAVIKEVLRL
HPPLPLLVPR LLSEDVKLKG YDITAGTQVI INAWAIQRDT ATWGSDAQEF RPERHFDSTW
DFVGRNFKYI PFGAGRRLCP GIGLGSVMAS VTLANLVKRF DWRVEDGPSG YDKPDLVEGA
GIDVCRKFPL VVFPSSA
