ID   C71AO_ARATH             Reviewed;         488 AA.
AC   Q9STK9; Q304B3;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 3.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Cytochrome P450 71A24;
DE            EC=1.14.-.-;
GN   Name=CYP71A24; OrderedLocusNames=At3g48290; ORFNames=T29H11.190;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9STK9-1; Sequence=Displayed;
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB41169.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL049659; CAB41169.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE78395.1; -; Genomic_DNA.
DR   EMBL; BT028956; ABI54331.1; -; mRNA.
DR   RefSeq; NP_680108.2; NM_148855.3. [Q9STK9-1]
DR   AlphaFoldDB; Q9STK9; -.
DR   SMR; Q9STK9; -.
DR   STRING; 3702.AT3G48290.2; -.
DR   MetOSite; Q9STK9; -.
DR   PRIDE; Q9STK9; -.
DR   ProteomicsDB; 240300; -. [Q9STK9-1]
DR   EnsemblPlants; AT3G48290.1; AT3G48290.1; AT3G48290. [Q9STK9-1]
DR   GeneID; 823987; -.
DR   Gramene; AT3G48290.1; AT3G48290.1; AT3G48290. [Q9STK9-1]
DR   KEGG; ath:AT3G48290; -.
DR   Araport; AT3G48290; -.
DR   eggNOG; KOG0156; Eukaryota.
DR   InParanoid; Q9STK9; -.
DR   OMA; NIRPRNE; -.
DR   PhylomeDB; Q9STK9; -.
DR   BioCyc; ARA:AT3G48290-MON; -.
DR   PRO; PR:Q9STK9; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9STK9; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..488
FT                   /note="Cytochrome P450 71A24"
FT                   /id="PRO_0000052074"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         433
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   488 AA;  56006 MW;  9A7DD3DE1461D892 CRC64;
     MKMMMMIILL LCSIILITIL FFKKQSRGKK SNAPPSPPRL PLIRNLHQLG RHPHRSLCSL
     SHRYGPLMLL HFGSVPVLVV SSADAAKDVL KTHDRVFASR PRSKIFDKIF YNGRDVALAP
     YGEYWRQMKS VCVLHLFSNK MVRSFRDVRQ EEISLMIEKI RISSSLRINL SEILVNLTNN
     VICRVALGRK YGGKTDFKDL MKRLTRLLGE FSVGSYVSWL AWIDWIRGLD GQLIKISNDL
     DEFLERVVQD HVDGDGHKND FVDFLLTIER EKSVGFEIDR LSIKAIILDV FVGDMDTTYT
     LLEWAMTELL CHHECLDRLQ EEVRMVCKDK SGVSEDDLQD MKYLKAVIKE TLRLHPPLPL
     MVPHESTHDV KLRDYHIPAG THVMINAWAI GREAATWGPD AEEFRPERHL NSYVDYRGQD
     TELVPFGAGR RICPAISFAV VLDEVVLANL VHQFDWTLPE ESTEYQTDVA ESTGMAVHRM
     FPLFAMTT