ID   TYLN_STRFR              Reviewed;         422 AA.
AC   O70023; Q7B309; Q9ZHQ5;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=O-mycaminosyltylonolide 6-deoxyallosyltransferase {ECO:0000303|PubMed:9651492};
DE            EC=2.4.1.317 {ECO:0000269|PubMed:9651492};
GN   Name=tylN {ECO:0000303|PubMed:9651492};
OS   Streptomyces fradiae (Streptomyces roseoflavus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1906;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=T59235;
RX   PubMed=9651492; DOI=10.1016/s0378-1119(98)00210-8;
RA   Wilson V.T., Cundliffe E.;
RT   "Characterization and targeted disruption of a glycosyltransferase gene in
RT   the tylosin producer, Streptomyces fradiae.";
RL   Gene 214:95-100(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=T59235;
RX   PubMed=10348045; DOI=10.7164/antibiotics.52.288;
RA   Wilson V.T., Cundliffe E.;
RT   "Molecular analysis of tlrB, an antibiotic-resistance gene from tylosin-
RT   producing Streptomyces fradiae, and discovery of a novel resistance
RT   mechanism.";
RL   J. Antibiot. 52:288-296(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10220165; DOI=10.1099/13500872-145-4-855;
RA   Fouces R., Mellado E., Diez B., Barredo J.L.;
RT   "The tylosin biosynthetic cluster from Streptomyces fradiae: genetic
RT   organization of the left region.";
RL   Microbiology 145:855-868(1999).
CC   -!- FUNCTION: Involved in the biosynthesis of the macrolide antibiotic
CC       tylosin derived from the polyketide lactone tylactone. Catalyzes the
CC       transfer of 6-deoxy-alpha-D-allose from dTDP-6-deoxy-alpha-D-allose to
CC       O-mycaminosyltylonolide (OMT) to yield demethyllactenocin.
CC       {ECO:0000269|PubMed:9651492}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-O-beta-D-mycaminosyltylonolide + dTDP-6-deoxy-alpha-D-allose
CC         = demethyllactenocin + dTDP + H(+); Xref=Rhea:RHEA:15357,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:58369, ChEBI:CHEBI:74143,
CC         ChEBI:CHEBI:76804, ChEBI:CHEBI:76810; EC=2.4.1.317;
CC         Evidence={ECO:0000269|PubMed:9651492};
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene produce demycinosyl-
CC       tylosin. {ECO:0000269|PubMed:9651492}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC       {ECO:0000305}.
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DR   EMBL; AJ005397; CAA06512.2; -; Genomic_DNA.
DR   EMBL; AJ009971; CAB37346.2; -; Genomic_DNA.
DR   EMBL; AF055922; AAD12163.1; -; Genomic_DNA.
DR   RefSeq; WP_043470782.1; NZ_MCNU01000172.1.
DR   AlphaFoldDB; O70023; -.
DR   SMR; O70023; -.
DR   STRING; 1906.SFRA_26475; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   KEGG; ag:CAA06512; -.
DR   PATRIC; fig|1906.11.peg.6042; -.
DR   eggNOG; COG1819; Bacteria.
DR   BioCyc; MetaCyc:MON-18390; -.
DR   BRENDA; 2.4.1.317; 5932.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:InterPro.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0030259; P:lipid glycosylation; IEA:InterPro.
DR   GO; GO:0033072; P:vancomycin biosynthetic process; IEA:UniProt.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR004276; GlycoTrans_28_N.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF03033; Glyco_transf_28; 1.
DR   Pfam; PF00201; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Glycosyltransferase; Transferase.
FT   CHAIN           1..422
FT                   /note="O-mycaminosyltylonolide 6-deoxyallosyltransferase"
FT                   /id="PRO_0000430760"
SQ   SEQUENCE   422 AA;  46635 MW;  DF88A02C359DAFBB CRC64;
     MRIALLTMGS RGDVQPFVAL GTGLRARGHE VVLGAPEALR PLVEQAGLEY RATPGDPDGF
     FTMPEVVETL RRGPAMRDLM KALPPAPEEY DQEVLDRIER AGEGVDLVVH APLTVTTALG
     EPSTPWLSVN WWPNTSTWTF PAVESGQRRM GPLTPLYNRL THWRAEREDW GWRRAEVNEF
     RGRRGLPPFG KSSPLRRLGH PRPHLYPFSP SVLPKPRDWP GQCHVTGYWF WDQPGWRPSP
     ELEDFLADGE PPVLLTLGST WPVHRQEEMV EYAVAAARGA RRRLLLVGGP EGALPGDALR
     VPSADYSWLM PRTAAVVHHG GFGTTADAVR AGVPQVLVPV FADHPFWAAR LRRMGTAARP
     VPLARMNREA LAASVRTAVT DPAMAVRARR LGEAVAAERG VENACVLIEE WAETRTTAHT
     PG