C71AP_ARATH
ID C71AP_ARATH Reviewed; 490 AA.
AC Q9STK8;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Cytochrome P450 71A25;
DE EC=1.14.-.-;
GN Name=CYP71A25; OrderedLocusNames=At3g48280; ORFNames=T29H11.200;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AL049659; CAB41170.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78394.1; -; Genomic_DNA.
DR PIR; T06714; T06714.
DR RefSeq; NP_680107.1; NM_148854.3.
DR AlphaFoldDB; Q9STK8; -.
DR SMR; Q9STK8; -.
DR STRING; 3702.AT3G48280.1; -.
DR PaxDb; Q9STK8; -.
DR PRIDE; Q9STK8; -.
DR ProteomicsDB; 240490; -.
DR EnsemblPlants; AT3G48280.1; AT3G48280.1; AT3G48280.
DR GeneID; 823986; -.
DR Gramene; AT3G48280.1; AT3G48280.1; AT3G48280.
DR KEGG; ath:AT3G48280; -.
DR Araport; AT3G48280; -.
DR TAIR; locus:504955637; AT3G48280.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_1_1; -.
DR InParanoid; Q9STK8; -.
DR OMA; GRHTHRS; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; Q9STK8; -.
DR BioCyc; ARA:AT3G48280-MON; -.
DR PRO; PR:Q9STK8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9STK8; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000137; C:Golgi cis cisterna; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..490
FT /note="Cytochrome P450 71A25"
FT /id="PRO_0000052075"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 431
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 490 AA; 55784 MW; FB97BA80A4C1EA50 CRC64;
MMMMIILLWS IIFMTILFLK KQLSGKKGKT PPSPPGLPLI GNLHQLGRHT HRSLCDLSRR
YGPLMLLHLG RVPVLIVSSA DMAQEILKTH DQAFANRPRS KLSQKLLYNN RDVASAPYGE
YWRQMKSVCV IHLLSNKMVR SFRDVREEEI TLMMAKIRKS SSLPFNVSKV LECLTNDVIC
RVALGRKYGG ETDFKKLTDR LSELLGTFSI GSFVPWLAWV DWIRGWDAQL DKMGKDLDDF
FEKVVQDHED GDRRDGTDLI DALLRVKREK SPGFEIERVS IKAITLDVFV GGSDTSFTLL
EWAMTELLRH PKSLNRLQEE VRTICKGKSR VSEDDIQGMK YLKAVIKEAL RLHPPFPMMA
PHESTEDVKL RDYHIPAGTQ VMMNAWAIGR EVATWGPDAE EFKPERHLDT SVDFRGQNFE
LLPFGAGRRI CPAVSFAVVL NEVVLANLVH GFDWKLPEES KEDKTDVAES SGFSVHREFP
LYAVASPYLT
