C71AR_ARATH
ID C71AR_ARATH Reviewed; 499 AA.
AC O65438; F4JUT2;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 3.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Cytochrome P450 71A27;
DE EC=1.14.-.-;
GN Name=CYP71A27; OrderedLocusNames=At4g20240; ORFNames=F1C12.160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP CONCEPTUAL TRANSLATION.
RA Bak S., Paquette S.;
RL Unpublished observations (APR-2001).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AEE84289.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AEE84289.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA18249.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At4g20235 and At4g20240.; Evidence={ECO:0000305};
CC Sequence=CAA18249.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAB79024.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At4g20235 and At4g20240.; Evidence={ECO:0000305};
CC Sequence=CAB79024.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AL022224; CAA18249.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161552; CAB79024.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84289.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; NP_193757.3; NM_118143.4.
DR AlphaFoldDB; O65438; -.
DR SMR; O65438; -.
DR STRING; 3702.AT4G20240.1; -.
DR PaxDb; O65438; -.
DR PRIDE; O65438; -.
DR ProteomicsDB; 223853; -.
DR GeneID; 827771; -.
DR KEGG; ath:AT4G20240; -.
DR Araport; AT4G20240; -.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_1_1; -.
DR OrthoDB; 467733at2759; -.
DR PhylomeDB; O65438; -.
DR BioCyc; ARA:AT4G20240-MON; -.
DR PRO; PR:O65438; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O65438; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 3: Inferred from homology;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..499
FT /note="Cytochrome P450 71A27"
FT /id="PRO_0000052077"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 438
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 499 AA; 57087 MW; DD00BAFDF5C80E4D CRC64;
MEMILISLCL TTLLAFLFLK PLLKRITTTK PKLPPSPWRL PVIGNLHQLG PNPHRYLHSL
SLRYGPLMLL HFGRVPVLVV SCPDVTNDIM KTHDLKFANR PKSKAINIFM EGGRDIIFGP
YGEDWKSMKS LGVVHLLNNK MVRSFENLRE EEIKVMTEKL EEASSSSSSV NLSKLLMTLT
NDIICRITLG RKYNEEEGGI DIKNLVMTSS EFFGKFFFGD FIPSLAWIDW ISGIDDKMKD
INNKLDCFLD SMVQEHVDAD HKEPSDFIDM LLLIQKDKTK RFKFDRSDLI LILKDMFFSG
TATTASQLEW TMTELMRHPE CMKKLQDEIN SFSTHNLNVT EKEVEKMNYL HCVIKEGLRL
HPSGPLLFRL PSEDVQLKGY DISAGTHVII NAWALQRNPA IWGLDANEYR PERHFWYEFG
FQRTDSKFVP FGAGRRLCPG IGFSLVLSKL ALANLVKRFN WRLKVGPVGD DKPDLAEASG
IDVCRKFPLI VFPSIAHTH
