C71B2_ARATH
ID C71B2_ARATH Reviewed; 502 AA.
AC O65788; Q41953; Q8VZK5; Q9SAE2;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Cytochrome P450 71B2;
DE EC=1.14.-.-;
GN Name=CYP71B2; OrderedLocusNames=At1g13080; ORFNames=F3F19.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=9620263; DOI=10.1023/a:1005921406884;
RA Mizutani M., Ward E., Ohta D.;
RT "Cytochrome P450 superfamily in Arabidopsis thaliana: isolation of cDNAs,
RT differential expression, and RFLP mapping of multiple cytochromes P450.";
RL Plant Mol. Biol. 37:39-52(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 124-260 (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O65788-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O65788-2; Sequence=VSP_008998, VSP_008999;
CC -!- TISSUE SPECIFICITY: Expressed in root, leaf and influorescence stem of
CC 3-4 week plants. {ECO:0000269|PubMed:9620263}.
CC -!- DEVELOPMENTAL STAGE: Constant expression level found in 3-12 week old
CC leaves. Expression levels decrease during the light period, then
CC increase again during dark. After wounding, expression levels decrease.
CC {ECO:0000269|PubMed:9620263}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; D78605; BAA28537.1; -; mRNA.
DR EMBL; AC007357; AAD31061.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28967.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28968.1; -; Genomic_DNA.
DR EMBL; AY064051; AAL36407.1; -; mRNA.
DR EMBL; BT003811; AAO41864.1; -; mRNA.
DR EMBL; Z18072; CAA79095.2; -; mRNA.
DR PIR; H86264; H86264.
DR PIR; T52173; T52173.
DR RefSeq; NP_172767.1; NM_101178.4. [O65788-1]
DR RefSeq; NP_849653.1; NM_179322.1. [O65788-2]
DR AlphaFoldDB; O65788; -.
DR SMR; O65788; -.
DR STRING; 3702.AT1G13080.1; -.
DR PaxDb; O65788; -.
DR PRIDE; O65788; -.
DR ProteomicsDB; 239134; -. [O65788-1]
DR EnsemblPlants; AT1G13080.1; AT1G13080.1; AT1G13080. [O65788-1]
DR EnsemblPlants; AT1G13080.2; AT1G13080.2; AT1G13080. [O65788-2]
DR GeneID; 837865; -.
DR Gramene; AT1G13080.1; AT1G13080.1; AT1G13080. [O65788-1]
DR Gramene; AT1G13080.2; AT1G13080.2; AT1G13080. [O65788-2]
DR KEGG; ath:AT1G13080; -.
DR Araport; AT1G13080; -.
DR TAIR; locus:2031900; AT1G13080.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_1_1; -.
DR InParanoid; O65788; -.
DR OMA; VQECIGD; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; O65788; -.
DR BioCyc; ARA:AT1G13080-MON; -.
DR PRO; PR:O65788; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O65788; baseline and differential.
DR Genevisible; O65788; AT.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0010286; P:heat acclimation; IEP:TAIR.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..502
FT /note="Cytochrome P450 71B2"
FT /id="PRO_0000052080"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 444
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..118
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_008998"
FT VAR_SEQ 119..143
FT /note="YGEYWREVRKLAVIELFSSKKVQSF -> MLQPTKDGGERKTLLRFQRHHFR
FT TI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_008999"
FT CONFLICT 226..236
FT /note="GLGRFVDWLFQ -> WTWKICRFGCFH (in Ref. 5; CAA79095)"
FT /evidence="ECO:0000305"
FT CONFLICT 242
FT /note="N -> T (in Ref. 5; CAA79095)"
FT /evidence="ECO:0000305"
FT CONFLICT 247..256
FT /note="ELDAFYQHVI -> GLECFLSACD (in Ref. 5; CAA79095)"
FT /evidence="ECO:0000305"
FT CONFLICT 332
FT /note="E -> G (in Ref. 1; BAA28537)"
FT /evidence="ECO:0000305"
FT CONFLICT 347
FT /note="E -> V (in Ref. 1; BAA28537)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 502 AA; 57140 MW; AABBB4FDDDBFB82B CRC64;
MTILLCFFLV SLLTIVSSIF LKQNKTSKFN LPPSPSSLPI IGNLHHLAGL PHRCFHKLSI
KYGPLVFLRL GSVPVVVISS SEAAEAVLKT NDLECCSRPK TVGSGKLSYG FKDITFAPYG
EYWREVRKLA VIELFSSKKV QSFRYIREEE VDFVVKKVSE SALKQSPVDL SKTFFSLTAS
IICRVALGQN FNESGFVIDQ DRIEELVTES AEALGTFTFS DFFPGGLGRF VDWLFQRHKK
INKVFKELDA FYQHVIDDHL KPEGRKNQDI VTLILDMIDK QEDSDSFKLN MDNLKAIVMD
VFLAGIDTSA VTMIWAMTEL IRNPRVMKKA QESIRTTLGL KKERITEEDL GKVEYLNHIL
KETFRLHPAL PFVVPRETMS HIKIQGYDIP PKTQIQLNVW TIGRDPKRWN DPEEFNPERF
ANSSVDFRGQ HFDLLPFGSG RRICPGMPMA IASVELALMN LLYYFDWSMP DGTKGEDIDM
EEAGNISIVK KIPLQLVPVQ RY
