TYPH_MOUSE
ID TYPH_MOUSE Reviewed; 471 AA.
AC Q99N42;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Thymidine phosphorylase;
DE Short=TP;
DE EC=2.4.2.4;
DE AltName: Full=TdRPase;
GN Name=Tymp; Synonyms=Ecgf1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Ishikawa F., Toyoshima H., Miyazono K., Haraguchi M.;
RT "Mouse thymidine phosphorylase.";
RL Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the reversible phosphorolysis of thymidine. The
CC produced molecules are then utilized as carbon and energy sources or in
CC the rescue of pyrimidine bases for nucleotide synthesis (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=phosphate + thymidine = 2-deoxy-alpha-D-ribose 1-phosphate +
CC thymine; Xref=Rhea:RHEA:16037, ChEBI:CHEBI:17748, ChEBI:CHEBI:17821,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57259; EC=2.4.2.4;
CC -!- PATHWAY: Pyrimidine metabolism; dTMP biosynthesis via salvage pathway;
CC dTMP from thymine: step 1/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the thymidine/pyrimidine-nucleoside
CC phosphorylase family. {ECO:0000305}.
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DR EMBL; AB060274; BAB41208.1; -; mRNA.
DR CCDS; CCDS27747.1; -.
DR RefSeq; NP_612175.1; NM_138302.1.
DR AlphaFoldDB; Q99N42; -.
DR SMR; Q99N42; -.
DR STRING; 10090.ENSMUSP00000023285; -.
DR BindingDB; Q99N42; -.
DR ChEMBL; CHEMBL2606; -.
DR iPTMnet; Q99N42; -.
DR PhosphoSitePlus; Q99N42; -.
DR jPOST; Q99N42; -.
DR MaxQB; Q99N42; -.
DR PaxDb; Q99N42; -.
DR PeptideAtlas; Q99N42; -.
DR PRIDE; Q99N42; -.
DR ProteomicsDB; 298078; -.
DR Antibodypedia; 253; 873 antibodies from 36 providers.
DR DNASU; 72962; -.
DR Ensembl; ENSMUST00000023285; ENSMUSP00000023285; ENSMUSG00000022615.
DR GeneID; 72962; -.
DR KEGG; mmu:72962; -.
DR UCSC; uc007xgl.2; mouse.
DR CTD; 1890; -.
DR MGI; MGI:1920212; Tymp.
DR VEuPathDB; HostDB:ENSMUSG00000022615; -.
DR eggNOG; ENOG502QPRY; Eukaryota.
DR GeneTree; ENSGT00390000009250; -.
DR HOGENOM; CLU_025040_0_2_1; -.
DR InParanoid; Q99N42; -.
DR OMA; DVWRRMI; -.
DR OrthoDB; 1214417at2759; -.
DR PhylomeDB; Q99N42; -.
DR TreeFam; TF332198; -.
DR BRENDA; 2.4.2.4; 3474.
DR Reactome; R-MMU-73614; Pyrimidine salvage.
DR Reactome; R-MMU-73621; Pyrimidine catabolism.
DR SABIO-RK; Q99N42; -.
DR UniPathway; UPA00578; UER00638.
DR BioGRID-ORCS; 72962; 2 hits in 60 CRISPR screens.
DR ChiTaRS; Tymp; mouse.
DR PRO; PR:Q99N42; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q99N42; protein.
DR Bgee; ENSMUSG00000022615; Expressed in liver and 79 other tissues.
DR Genevisible; Q99N42; MM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004645; F:1,4-alpha-oligoglucan phosphorylase activity; TAS:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0016154; F:pyrimidine-nucleoside phosphorylase activity; IEA:InterPro.
DR GO; GO:0009032; F:thymidine phosphorylase activity; IMP:MGI.
DR GO; GO:0009887; P:animal organ morphogenesis; TAS:MGI.
DR GO; GO:0006935; P:chemotaxis; TAS:MGI.
DR GO; GO:0046074; P:dTMP catabolic process; IMP:MGI.
DR GO; GO:0000002; P:mitochondrial genome maintenance; ISO:MGI.
DR GO; GO:0006206; P:pyrimidine nucleobase metabolic process; IEA:InterPro.
DR GO; GO:0006213; P:pyrimidine nucleoside metabolic process; ISO:MGI.
DR GO; GO:1905333; P:regulation of gastric motility; ISO:MGI.
DR GO; GO:0031641; P:regulation of myelination; ISO:MGI.
DR GO; GO:0051969; P:regulation of transmission of nerve impulse; ISO:MGI.
DR Gene3D; 3.40.1030.10; -; 1.
DR Gene3D; 3.90.1170.30; -; 1.
DR InterPro; IPR000312; Glycosyl_Trfase_fam3.
DR InterPro; IPR017459; Glycosyl_Trfase_fam3_N_dom.
DR InterPro; IPR036320; Glycosyl_Trfase_fam3_N_dom_sf.
DR InterPro; IPR035902; Nuc_phospho_transferase.
DR InterPro; IPR036566; PYNP-like_C_sf.
DR InterPro; IPR013102; PYNP_C.
DR InterPro; IPR018090; Pyrmidine_PPas_bac/euk.
DR InterPro; IPR000053; Thymidine/pyrmidine_PPase.
DR PANTHER; PTHR10515; PTHR10515; 1.
DR Pfam; PF02885; Glycos_trans_3N; 1.
DR Pfam; PF00591; Glycos_transf_3; 1.
DR Pfam; PF07831; PYNP_C; 1.
DR PIRSF; PIRSF000478; TP_PyNP; 1.
DR SMART; SM00941; PYNP_C; 1.
DR SUPFAM; SSF47648; SSF47648; 1.
DR SUPFAM; SSF52418; SSF52418; 1.
DR SUPFAM; SSF54680; SSF54680; 1.
DR TIGRFAMs; TIGR02644; Y_phosphoryl; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Phosphoprotein; Reference proteome; Transferase.
FT CHAIN 1..471
FT /note="Thymidine phosphorylase"
FT /id="PRO_0000059047"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 102
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 188
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 203
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 207
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT MOD_RES 6
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q5FVR2"
SQ SEQUENCE 471 AA; 49336 MW; C6AC64FC004DFF7E CRC64;
MAAPGTPPPS ASGGGGGEPR QLPELIRLKR DGGHLREADI RNFVHAVIDG RAQDTQIGAM
LMAIRLQGMN LEETSVLTRA LAESGQQLEW PKAWHQQLVD KHSTGGVGDK VSLVLAPALA
ACGCKVPMIS GRSLGHTGGT LDKLESIPGF GVTQSPEQML HILEEVGCCI VGQSAKLVPA
DGILYAARDV TATVDSVPLI TASILSKKAV EGLSTLVVDV KFGGAAVFPD QEKARELAKM
LVRVGVSLGL KVAAALTAMD NPLGRSVGHT LEVEEALLCL DGAGPPDLRD LVIRLGGAIL
WISGQAETQD QGAARVAAAL DDGSARRRFQ LMLSAQGVDP GLAKALCSGS PTQRRQLLPH
AREQEELLAP ADGIVECVRA LPLARVLHDL GAGRSRAGQP IRPGVGAEVL VDVGQCLSRG
TPWLRVHLDG PALSSQQRRT LQGALVLSDR APFKVPSPFA ELVLPPTIAQ P
